Coculture methodologies for the study of Wnt signals

Kestutis Planutis, Marina Planutiene, Randall F. Holcombe

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


In vivo, responses to extracellular Wnt ligands are context dependent; the temporal characteristics and intensity of the signal are critical in determining the target cell response. In general, Wnt ligand-induced differentiation in mammalian cells requires several days of exposure. In order to better characterize Wnt-induced signaling in vitro, side-by-side and partitioned cocultures can be utilized. These methodologies closely mimic how Wnt signals are transmitted in the tumor microenvironment.

Original languageEnglish
Title of host publicationWnt Signaling
Subtitle of host publicationPathway Methods and Mammalian Models
EditorsElizabeth Vincan
Number of pages7
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Coculture
  • Temporal response
  • Tumor microenvironment


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