Co-localization of the retinoblastoma protein and the epstein-barr virus-encoded nuclear antigen EBNA-5

W. Q. Jiang; L. Szekely; V. Wendel-Hansen; N. Ringertz; G. Klein; A. Rosén

doi:10.1016/0014-4827(91)90438-Z

Co-localization of the retinoblastoma protein and the epstein-barr virus-encoded nuclear antigen EBNA-5

W. Q. Jiang, L. Szekely, V. Wendel-Hansen, N. Ringertz, G. Klein, A. Rosén

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen in transformed monkey cells (COS). The nuclear structures stained by aRB1C1 and EBNA-5 antibodies are distinct from the structures detected with antibodies against centromeric proteins and certain snRNP epitopes. EBNA-5/Rb-positive domains do not selectively react with antibodies against the La antigen known to associate with the small EBV-encoded nuclear RNA species designated as the EBERs.

Original language	English
Pages (from-to)	314-318
Number of pages	5
Journal	Experimental Cell Research
Volume	197
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(91)90438-Z
State	Published - Dec 1991
Externally published	Yes

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@article{16f5fd29a5aa415398e16a0c2b317dd6,

title = "Co-localization of the retinoblastoma protein and the epstein-barr virus-encoded nuclear antigen EBNA-5",

abstract = "A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen in transformed monkey cells (COS). The nuclear structures stained by aRB1C1 and EBNA-5 antibodies are distinct from the structures detected with antibodies against centromeric proteins and certain snRNP epitopes. EBNA-5/Rb-positive domains do not selectively react with antibodies against the La antigen known to associate with the small EBV-encoded nuclear RNA species designated as the EBERs.",

author = "Jiang, \{W. Q.\} and L. Szekely and V. Wendel-Hansen and N. Ringertz and G. Klein and A. Ros{\'e}n",

note = "Funding Information: This research was supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Karolinska Insti-tutet, and AB ASTRA and by NIH Grant 5 ROlCA5222502. We thank Gunilla Kjellstrom and Evi Mellqvist for excellent technical assistance and Marie Wahren for immunopurification of the La antiserum.",

year = "1991",

month = dec,

doi = "10.1016/0014-4827(91)90438-Z",

language = "English",

volume = "197",

pages = "314--318",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Co-localization of the retinoblastoma protein and the epstein-barr virus-encoded nuclear antigen EBNA-5

AU - Jiang, W. Q.

AU - Szekely, L.

AU - Wendel-Hansen, V.

AU - Ringertz, N.

AU - Klein, G.

AU - Rosén, A.

N1 - Funding Information: This research was supported by grants from the Swedish Cancer Society, the Swedish Medical Research Council, the Karolinska Insti-tutet, and AB ASTRA and by NIH Grant 5 ROlCA5222502. We thank Gunilla Kjellstrom and Evi Mellqvist for excellent technical assistance and Marie Wahren for immunopurification of the La antiserum.

PY - 1991/12

Y1 - 1991/12

N2 - A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen in transformed monkey cells (COS). The nuclear structures stained by aRB1C1 and EBNA-5 antibodies are distinct from the structures detected with antibodies against centromeric proteins and certain snRNP epitopes. EBNA-5/Rb-positive domains do not selectively react with antibodies against the La antigen known to associate with the small EBV-encoded nuclear RNA species designated as the EBERs.

AB - A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen in transformed monkey cells (COS). The nuclear structures stained by aRB1C1 and EBNA-5 antibodies are distinct from the structures detected with antibodies against centromeric proteins and certain snRNP epitopes. EBNA-5/Rb-positive domains do not selectively react with antibodies against the La antigen known to associate with the small EBV-encoded nuclear RNA species designated as the EBERs.

UR - https://www.scopus.com/pages/publications/0026346116

U2 - 10.1016/0014-4827(91)90438-Z

DO - 10.1016/0014-4827(91)90438-Z

M3 - Article

C2 - 1659990

AN - SCOPUS:0026346116

SN - 0014-4827

VL - 197

SP - 314

EP - 318

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Co-localization of the retinoblastoma protein and the epstein-barr virus-encoded nuclear antigen EBNA-5

Abstract

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