TY - JOUR
T1 - Cloning and characterization of mammalian SMC1 and SMC3 genes and proteins, components of the DNA recombination complexes RC-1
AU - Stursberg, S.
AU - Riwar, B.
AU - Jessberger, R.
N1 - Funding Information:
We thank Dr Alexandre T. Akhmedov and Dr Fraser McBlane for critical reading of the manuscript. The Basel Institute for Immunology is founded and supported by Hoffmann–LaRoche Inc., Basel, Switzerland.
PY - 1999/3/4
Y1 - 1999/3/4
N2 - Members of the evolutionary conserved Structural Maintenance of Chromosomes (SMC) protein family are involved in chromosome condensation and gene dosage compensation with the SMC2 and SMC4 subtypes, and sister chromatid cohesion with the SMC1 and SMC3 subtypes. The bovine recombination protein complex RC-1, which catalyzes DNA transfer reactions, contains two heterodimeric SMC polypeptides, the genes of which have now been cloned, sequenced, and classified as bovine (b)SMC1 and bSMC3. Both proteins display all the characteristic features of the SMC family. FISH analysis localized the mouse SMC3 gene to chromosome 19D2-D3. Mono- and polyclonal antibodies specific for either subtype detected high levels of protein expression in lymphoid tissues, lung, testis and ovary. No change in levels of bSMC1 and bSMC3 proteins occurred after X-ray or UV-light irradiation of various cell lines or primary cells, and the amounts of individual proteins and the heterodimer are roughly constant throughout the cell cycle. Immunofluorescence of mouse cells detected the SMC1 protein in foci associated with the chromatin. These loci dissolve and the SMC protein dissociates from the chromatin during M phase.
AB - Members of the evolutionary conserved Structural Maintenance of Chromosomes (SMC) protein family are involved in chromosome condensation and gene dosage compensation with the SMC2 and SMC4 subtypes, and sister chromatid cohesion with the SMC1 and SMC3 subtypes. The bovine recombination protein complex RC-1, which catalyzes DNA transfer reactions, contains two heterodimeric SMC polypeptides, the genes of which have now been cloned, sequenced, and classified as bovine (b)SMC1 and bSMC3. Both proteins display all the characteristic features of the SMC family. FISH analysis localized the mouse SMC3 gene to chromosome 19D2-D3. Mono- and polyclonal antibodies specific for either subtype detected high levels of protein expression in lymphoid tissues, lung, testis and ovary. No change in levels of bSMC1 and bSMC3 proteins occurred after X-ray or UV-light irradiation of various cell lines or primary cells, and the amounts of individual proteins and the heterodimer are roughly constant throughout the cell cycle. Immunofluorescence of mouse cells detected the SMC1 protein in foci associated with the chromatin. These loci dissolve and the SMC protein dissociates from the chromatin during M phase.
KW - Cell cycle
KW - DNA recombination
KW - Intracellular localization
KW - SMC genes
KW - Sister chromatid cohesion
UR - http://www.scopus.com/inward/record.url?scp=0033522135&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(99)00021-9
DO - 10.1016/S0378-1119(99)00021-9
M3 - Article
C2 - 10072753
AN - SCOPUS:0033522135
SN - 0378-1119
VL - 228
SP - 1
EP - 12
JO - Gene
JF - Gene
IS - 1-2
ER -