Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis

Hirohisa Nakata; Toshimitsu Matsui; Mitsuhiro Ito; Taizo Taniguchi; Yoko Naribayashi; Noriyuki Arima; Akira Nakamura; Yoshikazu Kinoshita; Kazuo Chihara; Shuyn Hosoda; Tsutomu Chiba

doi:10.1016/0006-291X(92)91317-J

Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis

Hirohisa Nakata
, Toshimitsu Matsui
, Mitsuhiro Ito
, Taizo Taniguchi
, Yoko Naribayashi
, Noriyuki Arima
, Akira Nakamura
, Yoshikazu Kinoshita
, Kazuo Chihara
, Shuyn Hosoda
, Tsutomu Chiba

Research output: Contribution to journal › Article › peer-review

122 Scopus citations

Abstract

We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.

Original language	English
Pages (from-to)	1151-1157
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	187
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91317-J
State	Published - 16 Sep 1992
Externally published	Yes

Access to Document

10.1016/0006-291X(92)91317-J

Cite this

@article{ed47c9bc0e874a25b01090a2a4bccb7d,

title = "Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis",

abstract = "We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7\% and 49.0\% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.",

author = "Hirohisa Nakata and Toshimitsu Matsui and Mitsuhiro Ito and Taizo Taniguchi and Yoko Naribayashi and Noriyuki Arima and Akira Nakamura and Yoshikazu Kinoshita and Kazuo Chihara and Shuyn Hosoda and Tsutomu Chiba",

year = "1992",

month = sep,

day = "16",

doi = "10.1016/0006-291X(92)91317-J",

language = "English",

volume = "187",

pages = "1151--1157",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis

AU - Nakata, Hirohisa

AU - Matsui, Toshimitsu

AU - Ito, Mitsuhiro

AU - Taniguchi, Taizo

AU - Naribayashi, Yoko

AU - Arima, Noriyuki

AU - Nakamura, Akira

AU - Kinoshita, Yoshikazu

AU - Chihara, Kazuo

AU - Hosoda, Shuyn

AU - Chiba, Tsutomu

PY - 1992/9/16

Y1 - 1992/9/16

N2 - We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.

AB - We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.

UR - https://www.scopus.com/pages/publications/0026788539

U2 - 10.1016/0006-291X(92)91317-J

DO - 10.1016/0006-291X(92)91317-J

M3 - Article

C2 - 1530611

AN - SCOPUS:0026788539

SN - 0006-291X

VL - 187

SP - 1151

EP - 1157

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis

Abstract

Access to Document

Fingerprint

Cite this