TY - JOUR
T1 - Cloning and characterization of gastrin receptor from ECL carcinoid tumor of Mastomys natalensis
AU - Nakata, Hirohisa
AU - Matsui, Toshimitsu
AU - Ito, Mitsuhiro
AU - Taniguchi, Taizo
AU - Naribayashi, Yoko
AU - Arima, Noriyuki
AU - Nakamura, Akira
AU - Kinoshita, Yoshikazu
AU - Chihara, Kazuo
AU - Hosoda, Shuyn
AU - Chiba, Tsutomu
PY - 1992/9/16
Y1 - 1992/9/16
N2 - We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.
AB - We report here the cDNA cloning of a putative gastrin receptor from enterochromaffin-like (ECL) carcinoid tumor of Mastomys natalensis. For this study, we used the polymerase chain reaction technique to amplify transmembrane domain sequences related to rat pancreatic cholecystokinin (CCK)-A receptor from the ECL tumor cDNA library. The amino acid sequence deduced from the cloned cDNA showed 85.7% and 49.0% identity to canine parietal cell gastrin receptor and rat pancreatic CCK-A receptor, respectively. Ligand binding studies using COS-7 cells transfected with the cDNA showed the same binding specificity for gastrin and CCK-8 as the gastrin receptor on the Mastomys carcinoid tumor membrane. Both gastrin and CCK-8 elevated free cytosolic calcium concentration in COS-7 cells expressing the cloned receptor. RNA blot analysis revealed the expression of the gastrin receptor in both Mastomys stomach and brain.
UR - http://www.scopus.com/inward/record.url?scp=0026788539&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(92)91317-J
DO - 10.1016/0006-291X(92)91317-J
M3 - Article
C2 - 1530611
AN - SCOPUS:0026788539
SN - 0006-291X
VL - 187
SP - 1151
EP - 1157
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -