Clonality analysis of childhood all in remission: No evidence of clonal hematopoiesis

Lambert Busque, Robert Ilaria, Ramana Tantravahi, Howard Weinstein, D. Gary Gilliland

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6 Scopus citations

Abstract

We studied 98 female patients in remission (2-240 months) from childhood ALL to determine the clonality status of their hematopoiesis. Thirty-one (31.6%) were heterozygous at the PGK locus for the BstX1 endonuclease restriction site, permitting X-linked clonality assays to be performed. Two patients were in relapse at the time of study and were excluded. We used the PGK-PCR clonality assay (PPCA) to analyze DNA from PMN and mononuclear cells of the remaining 29 female patients. All (29/29) patients demonstrated polyclonal hematopoiesis. These data show that remission from childhood ALL involves reestablishment of polyclonally derived hematopoiesis in all patients studied.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalLeukemia Research
Volume18
Issue number2
DOIs
StatePublished - Feb 1994
Externally publishedYes

Keywords

  • Childhood acute lymphoblastic leukemia
  • PCR
  • PGK
  • clonality
  • cytogenetics

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