Clonal assay of mast cell progenitors in semi-solid culture with a high seeding efficiency

H. Sakoda; H. Tezuka; K. Itoh; H. Sawada; Y. Yoshida; H. Uchino; K. J. Mori

Clonal assay of mast cell progenitors in semi-solid culture with a high seeding efficiency

H. Sakoda
, H. Tezuka
, K. Itoh
, H. Sawada
, Y. Yoshida
, H. Uchino
, K. J. Mori

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

In vitro clonal assay of murine mast cell progenitors was examined in semi-solid culture by using conditioned medium of a murine leukemic T-cell line (STIL-3) as the source of interleukin 3 (IL-3). In soft agar gel, mast cell colonies were formed with a seeding efficiency of 100-200 per 10⁵ bone marrow cells, which was much higher than previously reported data. Macrophages were distinguishable from mast cells by their characteristic 'foamy' appearance in toluidine blue and May-Grunwald-Giemsa stained preparations and by their capacity to phagocytize carbon particles. Thus, the present method may provide a sensitive assay technique for the quantitative evaluation of mast cell progenitors.

Original language	English
Pages (from-to)	335-341
Number of pages	7
Journal	Biomedicine and Pharmacotherapy
Volume	42
Issue number	5
State	Published - 1988
Externally published	Yes

Cite this

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title = "Clonal assay of mast cell progenitors in semi-solid culture with a high seeding efficiency",

abstract = "In vitro clonal assay of murine mast cell progenitors was examined in semi-solid culture by using conditioned medium of a murine leukemic T-cell line (STIL-3) as the source of interleukin 3 (IL-3). In soft agar gel, mast cell colonies were formed with a seeding efficiency of 100-200 per 105 bone marrow cells, which was much higher than previously reported data. Macrophages were distinguishable from mast cells by their characteristic 'foamy' appearance in toluidine blue and May-Grunwald-Giemsa stained preparations and by their capacity to phagocytize carbon particles. Thus, the present method may provide a sensitive assay technique for the quantitative evaluation of mast cell progenitors.",

author = "H. Sakoda and H. Tezuka and K. Itoh and H. Sawada and Y. Yoshida and H. Uchino and Mori, \{K. J.\}",

year = "1988",

language = "English",

volume = "42",

pages = "335--341",

journal = "Biomedicine and Pharmacotherapy",

issn = "0753-3322",

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TY - JOUR

T1 - Clonal assay of mast cell progenitors in semi-solid culture with a high seeding efficiency

AU - Sakoda, H.

AU - Tezuka, H.

AU - Itoh, K.

AU - Sawada, H.

AU - Yoshida, Y.

AU - Uchino, H.

AU - Mori, K. J.

PY - 1988

Y1 - 1988

N2 - In vitro clonal assay of murine mast cell progenitors was examined in semi-solid culture by using conditioned medium of a murine leukemic T-cell line (STIL-3) as the source of interleukin 3 (IL-3). In soft agar gel, mast cell colonies were formed with a seeding efficiency of 100-200 per 105 bone marrow cells, which was much higher than previously reported data. Macrophages were distinguishable from mast cells by their characteristic 'foamy' appearance in toluidine blue and May-Grunwald-Giemsa stained preparations and by their capacity to phagocytize carbon particles. Thus, the present method may provide a sensitive assay technique for the quantitative evaluation of mast cell progenitors.

AB - In vitro clonal assay of murine mast cell progenitors was examined in semi-solid culture by using conditioned medium of a murine leukemic T-cell line (STIL-3) as the source of interleukin 3 (IL-3). In soft agar gel, mast cell colonies were formed with a seeding efficiency of 100-200 per 105 bone marrow cells, which was much higher than previously reported data. Macrophages were distinguishable from mast cells by their characteristic 'foamy' appearance in toluidine blue and May-Grunwald-Giemsa stained preparations and by their capacity to phagocytize carbon particles. Thus, the present method may provide a sensitive assay technique for the quantitative evaluation of mast cell progenitors.

UR - https://www.scopus.com/pages/publications/0023767263

M3 - Article

C2 - 3191210

AN - SCOPUS:0023767263

SN - 0753-3322

VL - 42

SP - 335

EP - 341

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

IS - 5

ER -

Clonal assay of mast cell progenitors in semi-solid culture with a high seeding efficiency

Abstract

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