Abstract
In vitro clonal assay of murine mast cell progenitors was examined in semi-solid culture by using conditioned medium of a murine leukemic T-cell line (STIL-3) as the source of interleukin 3 (IL-3). In soft agar gel, mast cell colonies were formed with a seeding efficiency of 100-200 per 105 bone marrow cells, which was much higher than previously reported data. Macrophages were distinguishable from mast cells by their characteristic 'foamy' appearance in toluidine blue and May-Grunwald-Giemsa stained preparations and by their capacity to phagocytize carbon particles. Thus, the present method may provide a sensitive assay technique for the quantitative evaluation of mast cell progenitors.
| Original language | English |
|---|---|
| Pages (from-to) | 335-341 |
| Number of pages | 7 |
| Journal | Biomedicine and Pharmacotherapy |
| Volume | 42 |
| Issue number | 5 |
| State | Published - 1988 |
| Externally published | Yes |