Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study

Chao Wang, Robert L. Bruce, Elizabeth A. Duch, Jyotica V. Patel, Joshua T. Smith, Yann Astier, Evan G. Colgan, Qinghuang Lin, Gustavo Stolovitzky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

We fabricate nanofluidic devices, comprising diamond-shaped nanopillars and nanochannels as narrow as 30 nm using photolithographic techniques, and demonstrate successful translocation of long 1DNA (48.5 kbp) and T4 DNA (166 kbp) through these nanostructures. λ-DNA molecules can transit through 10 μm-long nanochannels in ∼50 ± 10 msec at ∼210 μm/sec without clogging, due to prestretching of the DNA molecules that results from geometrical confinement and straddling of the molecules around the nano-pillars. λ-DNA translocation speed can be linearly tuned from ∼300 to ∼900 μm/sec by electrophoresis with a mobility of (1.3 ± 0.15) × 10-4 cm2/(V · sec). Importantly, T4 DNA molecules translocated through channels as small as 30 nm, extending their length to 73.5 μm in the process, i.e. ∼100 % of its dyed contour length.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1347-1349
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Dna stretching
  • Nanofluidic channels
  • Nanopillars
  • Single molecule imaging
  • Translocation

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