Clinical applications of the rapid high-performance liquid chromatographic determination of serum cortisol

Sandro Locke; Filippo Porcelli; Max Rosen; Michael Feffer; Elizabeth Stoner; Maria I. New

doi:10.1016/S0021-9673(01)91677-2

Clinical applications of the rapid high-performance liquid chromatographic determination of serum cortisol

Sandro Locke
, Filippo Porcelli
, Max Rosen
, Michael Feffer
, Elizabeth Stoner
, Maria I. New

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We report a rapid and specific analytical method for the measurement of serum cortisol by reversed-phase high-performance liquid chromatography (HPLC). The method allows the diagnostic measurement of cortisol in emergency clinical circumstances of glucocorticoid deficiency or excess. Results obtained by HPLC are comparable to those by radioimmunoassay. The lower limit of sensitivity is 6 ng cortisol per ml of serum.

Original language	English
Pages (from-to)	377-382
Number of pages	6
Journal	Journal of Chromatography A
Volume	317
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(01)91677-2
State	Published - 28 Dec 1984
Externally published	Yes

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title = "Clinical applications of the rapid high-performance liquid chromatographic determination of serum cortisol",

abstract = "We report a rapid and specific analytical method for the measurement of serum cortisol by reversed-phase high-performance liquid chromatography (HPLC). The method allows the diagnostic measurement of cortisol in emergency clinical circumstances of glucocorticoid deficiency or excess. Results obtained by HPLC are comparable to those by radioimmunoassay. The lower limit of sensitivity is 6 ng cortisol per ml of serum.",

author = "Sandro Locke and Filippo Porcelli and Max Rosen and Michael Feffer and Elizabeth Stoner and New, \{Maria I.\}",

note = "Funding Information: This study was supportedi n part by USPHS, NIH grant awardsH D 00072, HD 15074H, L 17749a nd AM 6354,b y granta wardR R 47 from theG eneralC linical ResearchC entersP rogram, Division of ResearchR esources,N IH, and by a grant from the Bristol-MeyersF und.",

year = "1984",

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doi = "10.1016/S0021-9673(01)91677-2",

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T1 - Clinical applications of the rapid high-performance liquid chromatographic determination of serum cortisol

AU - Locke, Sandro

AU - Porcelli, Filippo

AU - Rosen, Max

AU - Feffer, Michael

AU - Stoner, Elizabeth

AU - New, Maria I.

N1 - Funding Information: This study was supportedi n part by USPHS, NIH grant awardsH D 00072, HD 15074H, L 17749a nd AM 6354,b y granta wardR R 47 from theG eneralC linical ResearchC entersP rogram, Division of ResearchR esources,N IH, and by a grant from the Bristol-MeyersF und.

PY - 1984/12/28

Y1 - 1984/12/28

N2 - We report a rapid and specific analytical method for the measurement of serum cortisol by reversed-phase high-performance liquid chromatography (HPLC). The method allows the diagnostic measurement of cortisol in emergency clinical circumstances of glucocorticoid deficiency or excess. Results obtained by HPLC are comparable to those by radioimmunoassay. The lower limit of sensitivity is 6 ng cortisol per ml of serum.

AB - We report a rapid and specific analytical method for the measurement of serum cortisol by reversed-phase high-performance liquid chromatography (HPLC). The method allows the diagnostic measurement of cortisol in emergency clinical circumstances of glucocorticoid deficiency or excess. Results obtained by HPLC are comparable to those by radioimmunoassay. The lower limit of sensitivity is 6 ng cortisol per ml of serum.

UR - https://www.scopus.com/pages/publications/0021647734

U2 - 10.1016/S0021-9673(01)91677-2

DO - 10.1016/S0021-9673(01)91677-2

M3 - Article

C2 - 6530445

AN - SCOPUS:0021647734

SN - 0021-9673

VL - 317

SP - 377

EP - 382

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - C

ER -

Clinical applications of the rapid high-performance liquid chromatographic determination of serum cortisol

Abstract

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