CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis

Zeynep Cansu Uretmen Kagiali, Nazan Saner, Mehmet Akdag, Erdem Sanal, Beste Senem Degirmenci, Gurkan Mollaoglu, Nurhan Ozlu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

CLIC4 and CLIC1 are members of the well-conserved chloride intracellular channel proteins (CLICs) structurally related to glutathione-S-transferases. Here, we report new roles of CLICs in cytokinesis. At the onset of cytokinesis, CLIC4 accumulates at the cleavage furrow and later localizes to the midbody in a RhoA-dependent manner. The cell cycle–dependent localization of CLIC4 is abolished when its glutathione S-transferase activity–related residues (C35A and F37D) are mutated. Ezrin, anillin, and ALIX are identified as interaction partners of CLIC4 at the cleavage furrow and midbody. Strikingly, CLIC4 facilitates the activation of ezrin at the cleavage furrow and reciprocally inhibition of ezrin activation diminishes the translocation of CLIC4 to the cleavage furrow. Furthermore, knockouts of CLIC4 and CLIC1 cause abnormal blebbing at the polar cortex and regression of the cleavage furrow at late cytokinesis leading to multinucleated cells. We conclude that CLIC4 and CLIC1 function together with ezrin where they bridge plasma membrane and actin cytoskeleton at the polar cortex and cleavage furrow to promote cortical stability and successful completion of cytokinesis in mammalian cells.

Original languageEnglish
Article numbere201900558
JournalLife Science Alliance
Volume3
Issue number2
DOIs
StatePublished - 2020
Externally publishedYes

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