@inbook{32c2ac6ce531403fac09d05c35e49a26,
title = "Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin",
abstract = "Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.",
keywords = "CUT&RUN, Epidermis, Epigenetics, Histone modifications, Stem cells",
author = "Pooja Flora and Elena Ezhkova",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2023.",
year = "2024",
doi = "10.1007/7651_2023_499",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "9--21",
booktitle = "Methods in Molecular Biology",
}