Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis

Jun Xiao, Run Jin, Xiang Yu, Max Shen, John D. Wagner, Armaan Pai, Claire Song, Michael Zhuang, Samantha Klasfeld, Chongsheng He, Alexandre M. Santos, Chris Helliwell, Jose L. Pruneda-Paz, Steve A. Kay, Xiaowei Lin, Sujuan Cui, Meilin Fernandez Garcia, Oliver Clarenz, Justin Goodrich, Xiaoyu ZhangRyan S. Austin, Roberto Bonasio, Doris Wagner

Research output: Contribution to journalArticlepeer-review

180 Scopus citations


Disruption of gene silencing by Polycomb protein complexes leads to homeotic transformations and altered developmental-phase identity in plants1-5. Here we define short genomic fragments, known as Polycomb response elements (PREs), that direct Polycomb repressive complex 2 (PRC2) placement at developmental genes regulated by silencing in Arabidopsis thaliana. We identify transcription factor families that bind to these PREs, colocalize with PRC2 on chromatin, physically interact with and recruit PRC2, and are required for PRC2-mediated gene silencing in vivo. Two of the cis sequence motifs enriched in the PREs are cognate binding sites for the identified transcription factors and are necessary and sufficient for PRE activity. Thus PRC2 recruitment in Arabidopsis relies in large part on binding of trans-acting factors to cis-localized DNA sequence motifs.

Original languageEnglish
Pages (from-to)1546-1552
Number of pages7
JournalNature Genetics
Issue number10
StatePublished - 1 Oct 2017
Externally publishedYes


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