Circulating T Follicular Helper Subsets in Human Blood

Yayoi Iwamoto, Hideki Ueno

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations


CXCR5+ memory CD4+ T cells in human blood represent a circulating compartment of T follicular helper (Tfh) cells (termed circulating Tfh: cTfh cells). cTfh cells are composed of subsets that differ in phenotype and functions, and include resting and activated cell populations. Analysis of cTfh cell subsets have proven to be valuable to assess the ongoing global Tfh cell responses in vivo in distinct medical conditions including autoimmune diseases, infectious diseases, and after vaccination. Herein we will describe a detailed protocol to analyze cTfh subsets in human blood samples and to assess their helper capacity in culture with B cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Circulating T follicular helper cells
  • Human
  • Subsets


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