Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells

Carmit Bar, V. Julian Valdes, Elena Ezhkova

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a method designed to detect interactions between chromatin and the proteins bound to it. This method has been widely used for characterizing epigenetic landscapes in many cell types; however, a limiting factor has been the requirement of a high number of cells. Here, we describe a protocol for ChIP in epidermal cells from a newborn mouse, purified by fluorescence-activated cell sorting (FACS). This protocol has been optimized specifically for prefixed, low cell numbers, resulting in enough immunoprecipitated DNA suitable for genome-wide analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages19
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • ChIP-seq
  • Epidermal cells
  • FACS purified cells
  • Low cell number ChIP


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