@inbook{a56eeb63b56b40ab99d86493f6dd61aa,
title = "Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells",
abstract = "Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a method designed to detect interactions between chromatin and the proteins bound to it. This method has been widely used for characterizing epigenetic landscapes in many cell types; however, a limiting factor has been the requirement of a high number of cells. Here, we describe a protocol for ChIP in epidermal cells from a newborn mouse, purified by fluorescence-activated cell sorting (FACS). This protocol has been optimized specifically for prefixed, low cell numbers, resulting in enough immunoprecipitated DNA suitable for genome-wide analysis.",
keywords = "ChIP-seq, Epidermal cells, FACS purified cells, Low cell number ChIP",
author = "Carmit Bar and Valdes, {V. Julian} and Elena Ezhkova",
note = "Funding Information: We thank members of the Ezhkova and Valdes labs for discussion and suggestions. E.E. is supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under award number R01AR069078 and the Tisch Cancer Institute P30 Cancer Support Grant. C.B. is a Merksamer Fund scholar. J.V. is supported by the PAPIIT-UNAM IA202118, CONACYT 284867 and SECTEI/277/2019 grants. Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-0716-0648-3_17",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "197--215",
booktitle = "Methods in Molecular Biology",
}