@article{3e8df4e8c7994fd3885f4e9c1efbe6eb,
title = "Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin-binding neuroregulatory factors",
abstract = "Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, -GlcUA-GalNAc(4,6-O-disulfate)-, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.",
keywords = "Alzheimer's disease, Amyloid precursor protein, Appican, Chondroitin sulfate, Heparin-binding growth factor",
author = "Yuko Umehara and Shuhei Yamada and Shuji Nishimura and Junichi Shioi and Robakis, \{Nikolaos K.\} and Kazuyuki Sugahara",
note = "Funding Information: The authors thank Dr. Spiros Efthimiopoulos for a construction of His-tagged L-AβPP and Hitian Wang for appican purification. This work at Kobe Pharmaceutical University was supported in part by the Science Research Promotion Fund from the Japan Private School Promotion Foundation, and Grants-in-Aid for Scientific Research (B) 12557214 and Exploratory Research 15659021 from the Ministry of Education, Science, Culture, and Sports of Japan. The work at Mount Sinai School of Medicine was supported by the American Health Assistance Foundation (to J.S.) and NIH Grant AG05138 (to N.K.R.).",
year = "2004",
month = jan,
day = "16",
doi = "10.1016/S0014-5793(03)01506-0",
language = "English",
volume = "557",
pages = "233--238",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "1-3",
}