Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny

S. Huber; G. Braun; B. Schroppel; M. Horster

doi:10.1046/j.1523-1755.1998.06730.x

Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny

S. Huber, G. Braun, B. Schroppel, M. Horster

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down- regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.

Original language	English
Pages (from-to)	S149-S151
Journal	Kidney International, Supplement
Volume	54
Issue number	67
DOIs	https://doi.org/10.1046/j.1523-1755.1998.06730.x
State	Published - 1998
Externally published	Yes

Keywords

Collecting duct
Epithelia
Ion channels
Reverse transcriptase-polymerase chain reaction
Ureteric bud
β-actin

Access to Document

10.1046/j.1523-1755.1998.06730.x

Cite this

@article{4b1194a608ad406e8ead63f83dc54808,

title = "Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny",

abstract = "Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down- regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.",

keywords = "Collecting duct, Epithelia, Ion channels, Reverse transcriptase-polymerase chain reaction, Ureteric bud, β-actin",

author = "S. Huber and G. Braun and B. Schroppel and M. Horster",

note = "Funding Information: This work was supported by the Friedrich-Baur-Stiftung and the Deutsche Forschungsgemeinschaft (Ho 485/15-3).",

year = "1998",

doi = "10.1046/j.1523-1755.1998.06730.x",

language = "English",

volume = "54",

pages = "S149--S151",

journal = "Kidney International, Supplement",

issn = "0098-6577",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "67",

}

TY - JOUR

T1 - Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny

AU - Huber, S.

AU - Braun, G.

AU - Schroppel, B.

AU - Horster, M.

N1 - Funding Information: This work was supported by the Friedrich-Baur-Stiftung and the Deutsche Forschungsgemeinschaft (Ho 485/15-3).

PY - 1998

Y1 - 1998

N2 - Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down- regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.

AB - Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down- regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.

KW - Collecting duct

KW - Epithelia

KW - Ion channels

KW - Reverse transcriptase-polymerase chain reaction

KW - Ureteric bud

KW - β-actin

UR - http://www.scopus.com/inward/record.url?scp=0031754002&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.1998.06730.x

DO - 10.1046/j.1523-1755.1998.06730.x

M3 - Article

C2 - 9736273

AN - SCOPUS:0031754002

SN - 0098-6577

VL - 54

SP - S149-S151

JO - Kidney International, Supplement

JF - Kidney International, Supplement

IS - 67

ER -

Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny

Abstract

Keywords

Access to Document

Fingerprint

Cite this