Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant

E. Crisci; H. Almanza; I. Mena; L. Córdoba; E. Gómez-Casado; J. R. Castón; L. Fraile; J. Bárcena; M. Montoya

doi:10.1016/j.virol.2009.02.045

Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant

E. Crisci, H. Almanza, I. Mena, L. Córdoba, E. Gómez-Casado, J. R. Castón, L. Fraile, J. Bárcena, M. Montoya

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T cell epitopes. To accomplish this goal, we generated chimeric RHDV-VLPs incorporating a CD8⁺ T cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro, the chimeric VLPs activated dendritic cells for TNF-α secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein.

Original language	English
Pages (from-to)	303-312
Number of pages	10
Journal	Virology
Volume	387
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2009.02.045
State	Published - 10 May 2009
Externally published	Yes

Keywords

Dendritic cells
RHDV virus-like particles
Vaccine vector
Vaccinia virus infection

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10.1016/j.virol.2009.02.045

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@article{d82370929d204eeeb50b005086f3c63d,

title = "Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant",

abstract = "We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T cell epitopes. To accomplish this goal, we generated chimeric RHDV-VLPs incorporating a CD8+ T cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro, the chimeric VLPs activated dendritic cells for TNF-α secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein.",

keywords = "Dendritic cells, RHDV virus-like particles, Vaccine vector, Vaccinia virus infection",

author = "E. Crisci and H. Almanza and I. Mena and L. C{\'o}rdoba and E. G{\'o}mez-Casado and Cast{\'o}n, {J. R.} and L. Fraile and J. B{\'a}rcena and M. Montoya",

note = "Funding Information: This work was funded by grants: AGL2006-13809-C02-01, AGL2006-13809-C02-02 and CSD 2006-00007 (PORCIVIR, program CONSOLIDER-INGENIO 2010) from P.N. CICYT, and by the EU Network of Excellence, EPIZONE (Contract No FOOD-CT-2006-016236). E. Crisci is a recipient of a fellowship from Spanish Ministry of Science and Innovation (MICINN, FPI grants), and H. Almanza is a recipient of a fellowship from CONACYT (Mexico). I. Mena holds a contract from the “Ram{\'o}n y Cajal” program (MICINN).",

year = "2009",

month = may,

day = "10",

doi = "10.1016/j.virol.2009.02.045",

language = "English",

volume = "387",

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journal = "Virology",

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publisher = "Academic Press Inc.",

number = "2",

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T1 - Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant

AU - Crisci, E.

AU - Almanza, H.

AU - Mena, I.

AU - Córdoba, L.

AU - Gómez-Casado, E.

AU - Castón, J. R.

AU - Fraile, L.

AU - Bárcena, J.

AU - Montoya, M.

N1 - Funding Information: This work was funded by grants: AGL2006-13809-C02-01, AGL2006-13809-C02-02 and CSD 2006-00007 (PORCIVIR, program CONSOLIDER-INGENIO 2010) from P.N. CICYT, and by the EU Network of Excellence, EPIZONE (Contract No FOOD-CT-2006-016236). E. Crisci is a recipient of a fellowship from Spanish Ministry of Science and Innovation (MICINN, FPI grants), and H. Almanza is a recipient of a fellowship from CONACYT (Mexico). I. Mena holds a contract from the “Ramón y Cajal” program (MICINN).

PY - 2009/5/10

Y1 - 2009/5/10

N2 - We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T cell epitopes. To accomplish this goal, we generated chimeric RHDV-VLPs incorporating a CD8+ T cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro, the chimeric VLPs activated dendritic cells for TNF-α secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein.

AB - We have analyzed the potential of virus-like particles (VLPs) from rabbit hemorrhagic disease virus (RHDV) as a delivery system for foreign T cell epitopes. To accomplish this goal, we generated chimeric RHDV-VLPs incorporating a CD8+ T cell epitope (SIINFEKL) derived from chicken ovalbumin (OVA). The OVA epitope was inserted in the capsid protein (VP60) of RHDV at two different locations: 1) the N-terminus, predicted to be facing to the inner core of the VLPs, and 2) a novel insertion site predicted to be located within an exposed loop. Both constructions correctly assembled into VLPs. In vitro, the chimeric VLPs activated dendritic cells for TNF-α secretion and they were processed and presented to specific T cells. In vivo, mice immunized with the chimeric VLPs without adjuvant were able to induce specific cellular responses mediated by cytotoxic and memory T cells. More importantly, immunization with chimeric VLPs was able to resolve an infection by a recombinant vaccinia virus expressing OVA protein.

KW - Dendritic cells

KW - RHDV virus-like particles

KW - Vaccine vector

KW - Vaccinia virus infection

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DO - 10.1016/j.virol.2009.02.045

M3 - Article

C2 - 19327809

AN - SCOPUS:64849087010

SN - 0042-6822

VL - 387

SP - 303

EP - 312

JO - Virology

JF - Virology

IS - 2

ER -

Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant

Abstract

Keywords

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