CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2

Yong Hu, Xiaokun Liu, Anding Zhang, Hongbo Zhou, Ziduo Liu, Huanchun Chen, Meilin Jin

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

NS2 from influenza A virus mediates Crm1- dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2-Crm1 interaction, there is no doubt that NES1 is crucial for vRNP nuclear export. While the mechanism of the NES1 is still unclear, it is speculated that certain host partners might mediate the NES1 function through their interaction with NES1. In the present study, chromodomain-helicase-DNAbinding protein 3 (CHD3) was identified as a novel host nuclear protein for locating NS2 and Crm1 on dense chromatin for NS2 and Crm1-dependent vRNP nuclear export. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. Further, the knockdown of CHD3 would affect the propagation of the wild-type virus but not the mutant with the weakened NS2-CHD3 interaction. Therefore, this study demonstrates that NES1 is required for maximal binding of NS2 to CHD3, and that the NS2- CHD3 interaction on the dense chromatin contributed to the NS2-mediated vRNP nuclear export.

Original languageEnglish
Pages (from-to)971-982
Number of pages12
JournalCellular and Molecular Life Sciences
Volume72
Issue number5
DOIs
StatePublished - 1 Mar 2015
Externally publishedYes

Keywords

  • CHD3
  • Dense chromatin
  • Influenza A virus
  • VRNPs export

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