Characterizing the blood-brain barrier and gut barrier with super-resolution imaging: Opportunities and challenges

Ellen Doney, Renaud Bernatchez, Valérie Clavet-Fournier, Katarzyna A. Dudek, Laurence Dion-Albert, Flavie Lavoie-Cardinal, Caroline Menard

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Brain and gut barriers have been receiving increasing attention in health and diseases including in psychiatry. Recent studies have highlighted changes in the blood-brain barrier and gut barrier structural properties, notably a loss of tight junctions, leading to hyperpermeability, passage of inflammatory mediators, stress vulnerability, and the development of depressive behaviors. To decipher the cellular processes actively contributing to brain and gut barrier function in health and disease, scientists can take advantage of neurophotonic tools and recent advances in super-resolution microscopy techniques to complement traditional imaging approaches like confocal and electron microscopy. Here, we summarize the challenges, pros, and cons of these innovative approaches, hoping that a growing number of scientists will integrate them in their study design exploring barrier-related properties and mechanisms.

Original languageEnglish
Article number044410
JournalNeurophotonics
Volume10
Issue number4
DOIs
StatePublished - 1 Oct 2023
Externally publishedYes

Keywords

  • algorithms
  • stimulated emission depletion
  • stochastic optical reconstruction microscopy
  • tight junctions

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