Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS

Dongyue Xin; Leela Kurien; Katherine Briggs; Adrian Schimek; Richard Dambra; Daniel Hochdorfer; Tanja A. Arnouk; Marija Brgles; Saurabh Gautam; Dominik Hotter; Johannes Solzin; Thomas Kriehuber; Joseph Ashour; Adam Vigil; Michael Hawley; Xiaorong He

doi:10.1016/j.omtm.2025.101429

Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS

Dongyue Xin
, Leela Kurien
, Katherine Briggs
, Adrian Schimek
, Richard Dambra
, Daniel Hochdorfer
, Tanja A. Arnouk
, Marija Brgles
, Saurabh Gautam
, Dominik Hotter
, Johannes Solzin
, Thomas Kriehuber
, Joseph Ashour
, Adam Vigil
, Michael Hawley
, Xiaorong He

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Vesicular stomatitis virus expressing the glycoprotein of the lymphocytic choriomeningitis virus (VSV-GP) is a promising platform for oncolytic viruses and cancer vaccines. In this work, cryoelectron microscopy (cryo-EM) imaging was employed to directly visualize VSV-GP particles. Several different subpopulations of virus particle morphology were observed. Definition and fraction counting of subpopulations enabled quantitative comparison of subpopulation profiles between several VSV-GP samples. In developing an orthogonal method with higher throughput, we showed that the morphological profile of the VSV-GP particles can be characterized by size exclusion chromatography coupled with a multi-angle light scattering detector (SEC-MALS) based on a novel shape-based separation mechanism. Together, the two complementary techniques enable the analysis of morphological profile for VSV-GP and potentially other non-spherical viruses or nanoparticles.

Original language	English
Article number	101429
Journal	Molecular Therapy Methods and Clinical Development
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.omtm.2025.101429
State	Published - 13 Mar 2025
Externally published	Yes

Keywords

SEC-MALS
VSV-GP
analytical HPLC
cryo-EM
vesicular stomatitis virus
virus particle morphology

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10.1016/j.omtm.2025.101429

Cite this

Xin, D., Kurien, L., Briggs, K., Schimek, A., Dambra, R., Hochdorfer, D., Arnouk, T. A., Brgles, M., Gautam, S., Hotter, D., Solzin, J., Kriehuber, T., Ashour, J., Vigil, A., Hawley, M., & He, X. (2025). Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS. Molecular Therapy Methods and Clinical Development, 33(1), Article 101429. https://doi.org/10.1016/j.omtm.2025.101429

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title = "Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS",

abstract = "Vesicular stomatitis virus expressing the glycoprotein of the lymphocytic choriomeningitis virus (VSV-GP) is a promising platform for oncolytic viruses and cancer vaccines. In this work, cryoelectron microscopy (cryo-EM) imaging was employed to directly visualize VSV-GP particles. Several different subpopulations of virus particle morphology were observed. Definition and fraction counting of subpopulations enabled quantitative comparison of subpopulation profiles between several VSV-GP samples. In developing an orthogonal method with higher throughput, we showed that the morphological profile of the VSV-GP particles can be characterized by size exclusion chromatography coupled with a multi-angle light scattering detector (SEC-MALS) based on a novel shape-based separation mechanism. Together, the two complementary techniques enable the analysis of morphological profile for VSV-GP and potentially other non-spherical viruses or nanoparticles.",

keywords = "SEC-MALS, VSV-GP, analytical HPLC, cryo-EM, vesicular stomatitis virus, virus particle morphology",

author = "Dongyue Xin and Leela Kurien and Katherine Briggs and Adrian Schimek and Richard Dambra and Daniel Hochdorfer and Arnouk, \{Tanja A.\} and Marija Brgles and Saurabh Gautam and Dominik Hotter and Johannes Solzin and Thomas Kriehuber and Joseph Ashour and Adam Vigil and Michael Hawley and Xiaorong He",

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doi = "10.1016/j.omtm.2025.101429",

language = "English",

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Xin, D, Kurien, L, Briggs, K, Schimek, A, Dambra, R, Hochdorfer, D, Arnouk, TA, Brgles, M, Gautam, S, Hotter, D, Solzin, J, Kriehuber, T, Ashour, J, Vigil, A, Hawley, M & He, X 2025, 'Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS', Molecular Therapy Methods and Clinical Development, vol. 33, no. 1, 101429. https://doi.org/10.1016/j.omtm.2025.101429

TY - JOUR

T1 - Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS

AU - Xin, Dongyue

AU - Kurien, Leela

AU - Briggs, Katherine

AU - Schimek, Adrian

AU - Dambra, Richard

AU - Hochdorfer, Daniel

AU - Arnouk, Tanja A.

AU - Brgles, Marija

AU - Gautam, Saurabh

AU - Hotter, Dominik

AU - Solzin, Johannes

AU - Kriehuber, Thomas

AU - Ashour, Joseph

AU - Vigil, Adam

AU - Hawley, Michael

AU - He, Xiaorong

PY - 2025/3/13

Y1 - 2025/3/13

N2 - Vesicular stomatitis virus expressing the glycoprotein of the lymphocytic choriomeningitis virus (VSV-GP) is a promising platform for oncolytic viruses and cancer vaccines. In this work, cryoelectron microscopy (cryo-EM) imaging was employed to directly visualize VSV-GP particles. Several different subpopulations of virus particle morphology were observed. Definition and fraction counting of subpopulations enabled quantitative comparison of subpopulation profiles between several VSV-GP samples. In developing an orthogonal method with higher throughput, we showed that the morphological profile of the VSV-GP particles can be characterized by size exclusion chromatography coupled with a multi-angle light scattering detector (SEC-MALS) based on a novel shape-based separation mechanism. Together, the two complementary techniques enable the analysis of morphological profile for VSV-GP and potentially other non-spherical viruses or nanoparticles.

AB - Vesicular stomatitis virus expressing the glycoprotein of the lymphocytic choriomeningitis virus (VSV-GP) is a promising platform for oncolytic viruses and cancer vaccines. In this work, cryoelectron microscopy (cryo-EM) imaging was employed to directly visualize VSV-GP particles. Several different subpopulations of virus particle morphology were observed. Definition and fraction counting of subpopulations enabled quantitative comparison of subpopulation profiles between several VSV-GP samples. In developing an orthogonal method with higher throughput, we showed that the morphological profile of the VSV-GP particles can be characterized by size exclusion chromatography coupled with a multi-angle light scattering detector (SEC-MALS) based on a novel shape-based separation mechanism. Together, the two complementary techniques enable the analysis of morphological profile for VSV-GP and potentially other non-spherical viruses or nanoparticles.

KW - SEC-MALS

KW - VSV-GP

KW - analytical HPLC

KW - cryo-EM

KW - vesicular stomatitis virus

KW - virus particle morphology

UR - https://www.scopus.com/pages/publications/85218137620

U2 - 10.1016/j.omtm.2025.101429

DO - 10.1016/j.omtm.2025.101429

M3 - Article

AN - SCOPUS:85218137620

SN - 2329-0501

VL - 33

JO - Molecular Therapy Methods and Clinical Development

JF - Molecular Therapy Methods and Clinical Development

IS - 1

M1 - 101429

ER -

Characterization of VSV-GP morphology by cryo-EM imaging and SEC-MALS

Abstract

Keywords

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