Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses

T. M. Moran; M. Monestier; A. C.K. Lai; G. Norton; M. A. Reale; M. A. Thompson; J. L. Schulman; R. Riblet; C. A. Bona

doi:10.1089/vim.1987.1.1

Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses

T. M. Moran, M. Monestier, A. C.K. Lai, G. Norton, M. A. Reale, M. A. Thompson, J. L. Schulman, R. Riblet, C. A. Bona

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Abstract

Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H₃N₂) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H₃ and H₁). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (V(K))21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (V(H)) J558 or V(H) 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain.

Original language	English
Pages (from-to)	1-12
Number of pages	12
Journal	Viral Immunology
Volume	1
Issue number	1
DOIs	https://doi.org/10.1089/vim.1987.1.1
State	Published - 1987

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title = "Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses",

abstract = "Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (V(K))21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (V(H)) J558 or V(H) 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain.",

author = "Moran, {T. M.} and M. Monestier and Lai, {A. C.K.} and G. Norton and Reale, {M. A.} and Thompson, {M. A.} and Schulman, {J. L.} and R. Riblet and Bona, {C. A.}",

year = "1987",

doi = "10.1089/vim.1987.1.1",

language = "English",

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T1 - Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses

AU - Moran, T. M.

AU - Monestier, M.

AU - Lai, A. C.K.

AU - Norton, G.

AU - Reale, M. A.

AU - Thompson, M. A.

AU - Schulman, J. L.

AU - Riblet, R.

AU - Bona, C. A.

PY - 1987

Y1 - 1987

N2 - Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (V(K))21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (V(H)) J558 or V(H) 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain.

AB - Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (V(K))21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (V(H)) J558 or V(H) 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain.

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U2 - 10.1089/vim.1987.1.1

DO - 10.1089/vim.1987.1.1

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EP - 12

JO - Viral Immunology

JF - Viral Immunology

IS - 1

ER -

Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses

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