Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013

Chao liang Leng; Zhi jun Tian; Wu chao Zhang; Hong liang Zhang; Hong yue Zhai; Tong qing An; Jin mei Peng; Chao Ye; Long Sun; Qian Wang; Yan Sun; Lin Li; Hong yuan Zhao; Dan Chang; Xue hui Cai; Gui hong Zhang; Guang zhi Tong

doi:10.1016/j.vetmic.2014.03.005

Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013

Chao liang Leng, Zhi jun Tian, Wu chao Zhang, Hong liang Zhang, Hong yue Zhai, Tong qing An, Jin mei Peng, Chao Ye, Long Sun, Qian Wang, Yan Sun, Lin Li, Hong yuan Zhao, Dan Chang, Xue hui Cai, Gui hong Zhang, Guang zhi Tong

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Abstract

A newly emerged porcine reproductive and respiratory syndrome virus (PRRSV) that has caused severe reproductive losses in sows appeared in some regions of China in 2013. To explore the biology of this new PRRSV and understand more fully genetic diversity in PRRSV isolates from China, the complete genome of the two 2013 Chinese isolates, designated HLJA1 and HLJB1, were analyzed. Genomic sequence analysis showed that HLJA1 and HLJB1 shared 88.6-98.3% nucleotide identity with genotype 2 (North American type, NA-type) isolates, but only 61.1% with the genotype 1 (European type, EU-type) isolate of Lelystad virus, indicating that both these isolates belong to the NA-type PRRSV genotype. Phylogenetic analysis showed that the NA-type PRRSV isolates formed three subgroups (1, 2 and 3); representatives of these subgroups are VR-2332, CH-1a and HUN4, respectively. HLJA1 and HLJB1 belong to subgroup 2. Analysis of NSP2 revealed that HLJA1 has a 48-amino acid deletion at positions 473-480 and 482-521, unlike other HP-PRRSV isolates, while HLJB1 has only a 1-amino acid deletion at position 481 compared with CH-1a. Interestingly, HLJA1 replicated in PAM cells but not in MARC-145 cells, whereas HLJB1 replicated in both cell types. The neutralizing antibody titer of pig hyperimmune sera against HUN4 was significantly higher than that of HLJA1 or HLJB1. Additionally, genetic variability in GP5 and GP3 proteins and in the novel ORF5a protein was evident. In addition to elucidating the genetic relationships between PRRSV isolates, our results suggest that Chinese PRRSV will remain a pandemic virus.

Original language	English
Pages (from-to)	41-52
Number of pages	12
Journal	Veterinary Microbiology
Volume	171
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetmic.2014.03.005
State	Published - 2014
Externally published	Yes

Keywords

Genetic diversity
NSP2
ORF5a
PRRSV

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10.1016/j.vetmic.2014.03.005

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Leng, C. L., Tian, Z. J., Zhang, W. C., Zhang, H. L., Zhai, H. Y., An, T. Q., Peng, J. M., Ye, C., Sun, L., Wang, Q., Sun, Y., Li, L., Zhao, H. Y., Chang, D., Cai, X. H., Zhang, G. H., & Tong, G. Z. (2014). Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013. Veterinary Microbiology, 171(1-2), 41-52. https://doi.org/10.1016/j.vetmic.2014.03.005

@article{716d0fbbfe794ba5b692ce6e4eb7cd9d,

title = "Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013",

abstract = "A newly emerged porcine reproductive and respiratory syndrome virus (PRRSV) that has caused severe reproductive losses in sows appeared in some regions of China in 2013. To explore the biology of this new PRRSV and understand more fully genetic diversity in PRRSV isolates from China, the complete genome of the two 2013 Chinese isolates, designated HLJA1 and HLJB1, were analyzed. Genomic sequence analysis showed that HLJA1 and HLJB1 shared 88.6-98.3% nucleotide identity with genotype 2 (North American type, NA-type) isolates, but only 61.1% with the genotype 1 (European type, EU-type) isolate of Lelystad virus, indicating that both these isolates belong to the NA-type PRRSV genotype. Phylogenetic analysis showed that the NA-type PRRSV isolates formed three subgroups (1, 2 and 3); representatives of these subgroups are VR-2332, CH-1a and HUN4, respectively. HLJA1 and HLJB1 belong to subgroup 2. Analysis of NSP2 revealed that HLJA1 has a 48-amino acid deletion at positions 473-480 and 482-521, unlike other HP-PRRSV isolates, while HLJB1 has only a 1-amino acid deletion at position 481 compared with CH-1a. Interestingly, HLJA1 replicated in PAM cells but not in MARC-145 cells, whereas HLJB1 replicated in both cell types. The neutralizing antibody titer of pig hyperimmune sera against HUN4 was significantly higher than that of HLJA1 or HLJB1. Additionally, genetic variability in GP5 and GP3 proteins and in the novel ORF5a protein was evident. In addition to elucidating the genetic relationships between PRRSV isolates, our results suggest that Chinese PRRSV will remain a pandemic virus.",

keywords = "Genetic diversity, NSP2, ORF5a, PRRSV",

author = "Leng, {Chao liang} and Tian, {Zhi jun} and Zhang, {Wu chao} and Zhang, {Hong liang} and Zhai, {Hong yue} and An, {Tong qing} and Peng, {Jin mei} and Chao Ye and Long Sun and Qian Wang and Yan Sun and Lin Li and Zhao, {Hong yuan} and Dan Chang and Cai, {Xue hui} and Zhang, {Gui hong} and Tong, {Guang zhi}",

note = "Funding Information: This study was supported by the Heilongjiang Natural Science Funds for Distinguished Young Scholars (JC201314), the National Natural Science Foundation of China (grant no. 31001050 ) and the National High Technology Research and Development Program (863 plan) (grant no. 2011AA10A208).",

year = "2014",

doi = "10.1016/j.vetmic.2014.03.005",

language = "English",

volume = "171",

pages = "41--52",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier B.V.",

number = "1-2",

}

Leng, CL, Tian, ZJ, Zhang, WC, Zhang, HL, Zhai, HY, An, TQ, Peng, JM, Ye, C, Sun, L, Wang, Q, Sun, Y, Li, L, Zhao, HY, Chang, D, Cai, XH, Zhang, GH & Tong, GZ 2014, 'Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013', Veterinary Microbiology, vol. 171, no. 1-2, pp. 41-52. https://doi.org/10.1016/j.vetmic.2014.03.005

TY - JOUR

T1 - Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013

AU - Leng, Chao liang

AU - Tian, Zhi jun

AU - Zhang, Wu chao

AU - Zhang, Hong liang

AU - Zhai, Hong yue

AU - An, Tong qing

AU - Peng, Jin mei

AU - Ye, Chao

AU - Sun, Long

AU - Wang, Qian

AU - Sun, Yan

AU - Li, Lin

AU - Zhao, Hong yuan

AU - Chang, Dan

AU - Cai, Xue hui

AU - Zhang, Gui hong

AU - Tong, Guang zhi

N1 - Funding Information: This study was supported by the Heilongjiang Natural Science Funds for Distinguished Young Scholars (JC201314), the National Natural Science Foundation of China (grant no. 31001050 ) and the National High Technology Research and Development Program (863 plan) (grant no. 2011AA10A208).

PY - 2014

Y1 - 2014

N2 - A newly emerged porcine reproductive and respiratory syndrome virus (PRRSV) that has caused severe reproductive losses in sows appeared in some regions of China in 2013. To explore the biology of this new PRRSV and understand more fully genetic diversity in PRRSV isolates from China, the complete genome of the two 2013 Chinese isolates, designated HLJA1 and HLJB1, were analyzed. Genomic sequence analysis showed that HLJA1 and HLJB1 shared 88.6-98.3% nucleotide identity with genotype 2 (North American type, NA-type) isolates, but only 61.1% with the genotype 1 (European type, EU-type) isolate of Lelystad virus, indicating that both these isolates belong to the NA-type PRRSV genotype. Phylogenetic analysis showed that the NA-type PRRSV isolates formed three subgroups (1, 2 and 3); representatives of these subgroups are VR-2332, CH-1a and HUN4, respectively. HLJA1 and HLJB1 belong to subgroup 2. Analysis of NSP2 revealed that HLJA1 has a 48-amino acid deletion at positions 473-480 and 482-521, unlike other HP-PRRSV isolates, while HLJB1 has only a 1-amino acid deletion at position 481 compared with CH-1a. Interestingly, HLJA1 replicated in PAM cells but not in MARC-145 cells, whereas HLJB1 replicated in both cell types. The neutralizing antibody titer of pig hyperimmune sera against HUN4 was significantly higher than that of HLJA1 or HLJB1. Additionally, genetic variability in GP5 and GP3 proteins and in the novel ORF5a protein was evident. In addition to elucidating the genetic relationships between PRRSV isolates, our results suggest that Chinese PRRSV will remain a pandemic virus.

AB - A newly emerged porcine reproductive and respiratory syndrome virus (PRRSV) that has caused severe reproductive losses in sows appeared in some regions of China in 2013. To explore the biology of this new PRRSV and understand more fully genetic diversity in PRRSV isolates from China, the complete genome of the two 2013 Chinese isolates, designated HLJA1 and HLJB1, were analyzed. Genomic sequence analysis showed that HLJA1 and HLJB1 shared 88.6-98.3% nucleotide identity with genotype 2 (North American type, NA-type) isolates, but only 61.1% with the genotype 1 (European type, EU-type) isolate of Lelystad virus, indicating that both these isolates belong to the NA-type PRRSV genotype. Phylogenetic analysis showed that the NA-type PRRSV isolates formed three subgroups (1, 2 and 3); representatives of these subgroups are VR-2332, CH-1a and HUN4, respectively. HLJA1 and HLJB1 belong to subgroup 2. Analysis of NSP2 revealed that HLJA1 has a 48-amino acid deletion at positions 473-480 and 482-521, unlike other HP-PRRSV isolates, while HLJB1 has only a 1-amino acid deletion at position 481 compared with CH-1a. Interestingly, HLJA1 replicated in PAM cells but not in MARC-145 cells, whereas HLJB1 replicated in both cell types. The neutralizing antibody titer of pig hyperimmune sera against HUN4 was significantly higher than that of HLJA1 or HLJB1. Additionally, genetic variability in GP5 and GP3 proteins and in the novel ORF5a protein was evident. In addition to elucidating the genetic relationships between PRRSV isolates, our results suggest that Chinese PRRSV will remain a pandemic virus.

KW - Genetic diversity

KW - NSP2

KW - ORF5a

KW - PRRSV

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DO - 10.1016/j.vetmic.2014.03.005

M3 - Article

C2 - 24703221

AN - SCOPUS:84899974831

SN - 0378-1135

VL - 171

SP - 41

EP - 52

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013

Abstract

Keywords

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