TY - JOUR
T1 - Characterization of swine-origin H1N1 canine influenza viruses
AU - Wang, Guojun
AU - dos Anjos Borges, Luiz Gustavo
AU - Stadlbauer, Daniel
AU - Ramos, Irene
AU - Bermúdez González, Maria C.
AU - He, Jianqiao
AU - Ding, Yangbao
AU - Wei, Zuzhang
AU - Ouyang, Kang
AU - Huang, Weijian
AU - Simon, Viviana
AU - Fernandez-Sesma, Ana
AU - Krammer, Florian
AU - Nelson, Martha I.
AU - Chen, Ying
AU - García-Sastre, Adolfo
N1 - Funding Information:
The study was supported by [Inner Mongolia University Start-Up Fund] under Grant [No. 30500-5195126]; [Guangxi Natural Science Foundation] under Grant [No. 2016GXNSFBA380219]; [Guangxi Natural Science Foundation] under Grant [No. 2018GXNSFAA294048]; [Guangxi Science and Technology Bureau] under Grant [No. AA17204057]; [NIAID funded Center of Excellence for Influenza Surveillance and Research (CEIRS)] under Grant [No. HHSN272201400008C, to AG-S]. This study was also partially supported by CRIP (Center for Research on Influenza Pathogenesis).
Publisher Copyright:
© 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.
AB - Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.
KW - H1N1
KW - Host switch
KW - canine
KW - influenza A viruses
KW - reassortant
UR - http://www.scopus.com/inward/record.url?scp=85068684747&partnerID=8YFLogxK
U2 - 10.1080/22221751.2019.1637284
DO - 10.1080/22221751.2019.1637284
M3 - Article
C2 - 31287780
AN - SCOPUS:85068684747
SN - 2222-1751
VL - 8
SP - 1017
EP - 1026
JO - Emerging Microbes and Infections
JF - Emerging Microbes and Infections
IS - 1
ER -