@article{4bb63d17ee6047b4bd22842b84052d22,
title = "Characterization of stable complexes involving apolipoprotein E and the amyloid β peptide in Alzheimer's disease brain",
abstract = "Genetic evidence suggests a role for apolipoprotein E (apoE) in Alzheimer's disease (AD) amyloidogenesis. Here, amyloid-associated apoE from 32 AD patients was purified and characterized. We found that brain amyloid-associated apoE apparently exists not as free molecules but as complexes with polymers of the amyloid β peptide (Aβ). Brain Aβ-apoE complexes were detected irrespective of the apoE genotype, and similar complexes could be mimicked in vitro. The fine structure of purified Aβ-apoE complexes was fibrillar, and immunogold labeling revealed apoE immunoreactivity along the fibrils. Thus, we conclude that Aβ-apoE complexes are principal components of AD-associated brain amyloid and that the data presented here support a role for apoE in the pathogenesis of AD.",
author = "Jan N{\"a}slund and Johan Thyberg and Tjernberg, {Lars O.} and Christer Wernstedt and Karlstr{\"o}m, {Anders R.} and Nenad Bogdanovic and Gandy, {Samuel E.} and Lars Lannfelt and Lars Terenius and Christer Nordstedt",
note = "Funding Information: We thank Drs. T. Suzuki and T. Ramabhadran for providing antiserum G504 and monoclonal antibody 56-1, respectively; Drs. K. S. Kim and H. M. Wisniewski for providing ascites 6ElO and 4G6; and Dr. Yves L. Marcel, Lipoproteinand AtherosclerosisGroup, Universityof Ottawa Hear-l Institute, Ottawa, Ontario, Canada, for providing ascites 1 D7 and 3Hl. We also thank lnga Volkmann, Lena Lilius, and Karin Blom-gren for expert technical assistance. AD brain tissue was obtained from the Huddinge Brain Bank, Huddinge Hospital, Sweden. This study was supported by National Institutes of Health grants HL49571-01 (L. T.) and AG-11506 (S. E. G.), The Swedish Medical Research Council (J. T. and L.T.), The C. V. Starr Foundation and The Starr Program for Neurogeriatric Research (S. E. G.), The Swedish Heart Lung Foundation (J. T.), and the King Gustaf V 60th Birthday Fund (J. T.). J. N. is supported by a fellowship from The Swedish Board of Industrial Development. C. N. is a recipient of fellowships from The Berth von Kantzow Foundation, The Swedish Society for Medical Research, and The Nicholson Foundation.",
year = "1995",
month = jul,
doi = "10.1016/0896-6273(95)90079-9",
language = "English",
volume = "15",
pages = "219--228",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}