TY - JOUR
T1 - Characterization of in vivo-activated T cell clones from peripheral blood of multiple sclerosis patients
AU - Ofosu-Appiah, William
AU - Mokhtarian, Foroozan
AU - Miller, Aaron
AU - Grob, David
N1 - Funding Information:
We gratefully acknowledge the assistance of Drs. M. Keilson and D. Shirazian for obtaining the blood samples for the studies. Special thanks go to Ms. Linda Morgante for her valuable efforts in the follow up of MS patients for this study. This work was supported by Grants RG 1004-A-l from National Multiple Sclerosis Society and R29NS24688 from National Institutes of Health, and a grant from the Maimonides Research and Development Foundation.
PY - 1991/1
Y1 - 1991/1
N2 - In vivo-activated interleukin-2 responsive T cell clones were generated from peripheral blood (PBL) of multiple sclerosis patients (MS) and normal subjects (N) by limiting dilution analysis. The frequency with which interleukin-2 responsive cells were cloned from PBL was higher in MS than N. CD8 was the predominant phenotype expressed by both MS (85%) and N (89%) clones. Seven clones from four MS patients but none from five N subjects specifically proliferated against myelin basic protein. These studies demonstrate the existence of MBP-reactive T cells in PBL of MS patients.
AB - In vivo-activated interleukin-2 responsive T cell clones were generated from peripheral blood (PBL) of multiple sclerosis patients (MS) and normal subjects (N) by limiting dilution analysis. The frequency with which interleukin-2 responsive cells were cloned from PBL was higher in MS than N. CD8 was the predominant phenotype expressed by both MS (85%) and N (89%) clones. Seven clones from four MS patients but none from five N subjects specifically proliferated against myelin basic protein. These studies demonstrate the existence of MBP-reactive T cells in PBL of MS patients.
UR - https://www.scopus.com/pages/publications/0025634309
U2 - 10.1016/0090-1229(91)90147-3
DO - 10.1016/0090-1229(91)90147-3
M3 - Article
C2 - 1701373
AN - SCOPUS:0025634309
SN - 0090-1229
VL - 58
SP - 46
EP - 55
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 1
ER -