Characterization of human genomic yeast artificial chromosome inserts containing hexokinase 1 coding information on chromosome 10

Bruce D. Gelb, Kim C. Worley, Lisa D. Griffin, Volker Admas, A. Craig Chinault, Edward R.B. McCabe

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Hexokinase 1 (HK1) is one of four mammalian HK isoenzymes and maps to human chromosome 10. Two yeast artificial chromosomes (YACs) were identified in the Washington University human YAC library using polymerase chain reaction (PCR) primers designed with knowledge of the human HK1 cDNA sequence. YAC B129B12 is 120 kb in length and maps entirely to chromosome 10. YAC A159D5 is 400 kb in length and appears to have resulted from a recombination of chromosome 10 with non-chromosome 10 material. We report these YACs as potential resources for those interested in HK1 gene organization and mapping, as well as those desiring additional genomic information and markers on chromosome 10.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalBiochemical Medicine and Metabolic Biology
Volume47
Issue number3
DOIs
StatePublished - Jun 1992
Externally publishedYes

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