Characterization of a de novo unbalanced chromosome rearrangement by comparative genomic hybridization and fluorescence in situ hybridization

B. Levy, I. F. Gershin, R. J. Desnick, A. Babu, B. D. Gelb, K. Hirschhorn, P. D. Cotter

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Abstract

Fluorescence in situ hybridization (FISH) has proven useful for the identification of chromosomal material of unknown origin. More recently, comparative genomic hybridization (CGH) has been used to identify deletions and amplifications, particularly in neoplastic samples. Here, we describe the combined use of CGH and FISH to identify the origin of a de novo unbalanced translocation in a newborn with multiple congenital anomalies. GTG banding of metaphases from cultured lymphocytes showed an unbalanced karyotype, with extra material on a chromosome 5: 46, XX, add(5)(q35). Parental karyotypes were both normal. CGH revealed the additional material was from distal 1 1q (11q23->qter). This finding was confirmed by FISH with a whole chromosome paint for chromosome 11.Based on the CGH and FISH analyses, the pro-band’s karyotype wastherefore 46, XX, der(5)t(5;11)(q35.2; q23.2).ish der(5)(wcp11+).This case demonstrates the efficient use of CGH and confirmatory FISH for the identification of chromosomal material of unknown origin.

Original languageEnglish
Pages (from-to)68-71
Number of pages4
JournalCytogenetics and Cell Genetics
Volume76
Issue number1-2
DOIs
StatePublished - 1 Jan 1997
Externally publishedYes

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