TY - JOUR
T1 - Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan
AU - Naoi, Yuki
AU - Kishimoto, Mai
AU - Masuda, Tsuneyuki
AU - Ito, Mika
AU - Tsuchiaka, Shinobu
AU - Sano, Kaori
AU - Yamasato, Hiroshi
AU - Omatsu, Tsutomu
AU - Aoki, Hiroshi
AU - Furuya, Tetsuya
AU - Katayama, Yukie
AU - Oba, Mami
AU - Okada, Takashi
AU - Shirai, Junsuke
AU - Mizutani, Tetsuya
AU - Nagai, Makoto
N1 - Publisher Copyright:
© 2016, Springer-Verlag Wien.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - During an investigation of porcine fecal viruses using a metagenomics approach, a novel picornavirus was identified from the feces of a healthy two-month-old pig. This virus, named porcine picornavirus Japan (PPVJ), had a standard picornavirus genome organization, including the L protein region. The 5′ untranslated region harbored a type II internal ribosomal entry site. This virus was most closely related to lesavirus 1 (amino acid sequence identity: 38.2 %) in P1, equine rhinitis A virus (25.8 %) in P2, and lesavirus 2 (40.9 %) in P3. According to the genus demarcations for the family Picornaviridae (less than 40 %, 40 %, and 50 % amino acid sequence identity in P1, P2, and P3, respectively), PPVJ represents a new genus in the family Picornaviridae. PPVJ was detected in 23.3 % of the fecal samples (from 58.3 % of the farms across a wide area) from pigs less than four months old, by reverse transcription PCR, using specific primers designed from the 3D sequence, followed by sequencing. The host range and pathogenic potential of this virus in animals is yet to be determined.
AB - During an investigation of porcine fecal viruses using a metagenomics approach, a novel picornavirus was identified from the feces of a healthy two-month-old pig. This virus, named porcine picornavirus Japan (PPVJ), had a standard picornavirus genome organization, including the L protein region. The 5′ untranslated region harbored a type II internal ribosomal entry site. This virus was most closely related to lesavirus 1 (amino acid sequence identity: 38.2 %) in P1, equine rhinitis A virus (25.8 %) in P2, and lesavirus 2 (40.9 %) in P3. According to the genus demarcations for the family Picornaviridae (less than 40 %, 40 %, and 50 % amino acid sequence identity in P1, P2, and P3, respectively), PPVJ represents a new genus in the family Picornaviridae. PPVJ was detected in 23.3 % of the fecal samples (from 58.3 % of the farms across a wide area) from pigs less than four months old, by reverse transcription PCR, using specific primers designed from the 3D sequence, followed by sequencing. The host range and pathogenic potential of this virus in animals is yet to be determined.
UR - https://www.scopus.com/pages/publications/84961615818
U2 - 10.1007/s00705-016-2834-7
DO - 10.1007/s00705-016-2834-7
M3 - Article
C2 - 27016931
AN - SCOPUS:84961615818
SN - 0304-8608
VL - 161
SP - 1685
EP - 1690
JO - Archives of Virology
JF - Archives of Virology
IS - 6
ER -