Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells

M. S. Parshley; J. M. Cerreta; I. Mandl; J. A. Fierer; G. M. Turino

doi:10.1007/BF02618251

Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells

M. S. Parshley, J. M. Cerreta, I. Mandl, J. A. Fierer, G. M. Turino

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.

Original language	English
Pages (from-to)	709-722
Number of pages	14
Journal	In Vitro
Volume	15
Issue number	9
DOIs	https://doi.org/10.1007/BF02618251
State	Published - Sep 1979
Externally published	Yes

Keywords

endothelial clone
normal adult rat lung

Access to Document

10.1007/BF02618251

Cite this

@article{ee6249931c6a4eef8c1137bcd935404f,

title = "Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells",

abstract = "A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.",

keywords = "endothelial clone, normal adult rat lung",

author = "Parshley, {M. S.} and Cerreta, {J. M.} and I. Mandl and Fierer, {J. A.} and Turino, {G. M.}",

year = "1979",

month = sep,

doi = "10.1007/BF02618251",

language = "English",

volume = "15",

pages = "709--722",

journal = "In Vitro",

issn = "0073-5655",

publisher = "Springer New York",

number = "9",

}

TY - JOUR

T1 - Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells

AU - Parshley, M. S.

AU - Cerreta, J. M.

AU - Mandl, I.

AU - Fierer, J. A.

AU - Turino, G. M.

PY - 1979/9

Y1 - 1979/9

N2 - A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.

AB - A strain of endothelial cells derived from a single cell cloned from a line of normal adult rat lung parenchyma has been maintained in tissue culture for more than 3 years. These cells have been identified as endothelial cells based on the combination of their growth characteristics, cell morphology as observed with both light and electron microscopy, and their physiological properties. They have continued to produce granules, which stain specifically for glycosaminoglycans with Alcian blue, for over 2 1/2 years. During the same period of time, glycosaminoglycans were identified biochemically in both cells and medium. They have maintained the ability to degrade bradykinin over this period as well.

KW - endothelial clone

KW - normal adult rat lung

UR - http://www.scopus.com/inward/record.url?scp=0018569869&partnerID=8YFLogxK

U2 - 10.1007/BF02618251

DO - 10.1007/BF02618251

M3 - Article

C2 - 535916

AN - SCOPUS:0018569869

VL - 15

SP - 709

EP - 722

JO - In Vitro

JF - In Vitro

SN - 0073-5655

IS - 9

ER -

Characteristics of a clone of endothelial cells derived from a line of normal adult rat lung cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this