Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil

Luiz Gustavo Dos Anjos Borges; Virgínia Dalla Vechia; Gertrudes Corçóo

doi:10.1016/S0168-6496(03)00147-8

Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil

Luiz Gustavo Dos Anjos Borges
, Virgínia Dalla Vechia
, Gertrudes Corçóo

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feijó, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon-Weaver, H′=2.92) and site 3 exhibited the lowest diversity degree (Shannon-Weaver, H′=2.16).

Original language	English
Pages (from-to)	173-180
Number of pages	8
Journal	FEMS Microbiology Ecology
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/S0168-6496(03)00147-8
State	Published - 25 Jul 2003
Externally published	Yes

Keywords

Escherichia coli
Genetic characterization
Genetic diversity
Repetitive extragenic palindromic elements-polymerase chain reaction
Water isolate

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10.1016/S0168-6496(03)00147-8

Cite this

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title = "Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil",

abstract = "The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feij{\'o}, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70\% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon-Weaver, H′=2.92) and site 3 exhibited the lowest diversity degree (Shannon-Weaver, H′=2.16).",

keywords = "Escherichia coli, Genetic characterization, Genetic diversity, Repetitive extragenic palindromic elements-polymerase chain reaction, Water isolate",

author = "\{Dos Anjos Borges\}, \{Luiz Gustavo\} and \{Dalla Vechia\}, Virg{\'i}nia and Gertrudes Cor{\c c}{\'o}o",

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doi = "10.1016/S0168-6496(03)00147-8",

language = "English",

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TY - JOUR

T1 - Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil

AU - Dos Anjos Borges, Luiz Gustavo

AU - Dalla Vechia, Virgínia

AU - Corçóo, Gertrudes

PY - 2003/7/25

Y1 - 2003/7/25

N2 - The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feijó, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon-Weaver, H′=2.92) and site 3 exhibited the lowest diversity degree (Shannon-Weaver, H′=2.16).

AB - The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feijó, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon-Weaver, H′=2.92) and site 3 exhibited the lowest diversity degree (Shannon-Weaver, H′=2.16).

KW - Escherichia coli

KW - Genetic characterization

KW - Genetic diversity

KW - Repetitive extragenic palindromic elements-polymerase chain reaction

KW - Water isolate

UR - https://www.scopus.com/pages/publications/0038447169

U2 - 10.1016/S0168-6496(03)00147-8

DO - 10.1016/S0168-6496(03)00147-8

M3 - Article

AN - SCOPUS:0038447169

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JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 2

ER -

Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil

Abstract

Keywords

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