@inbook{1c1abb5dc782450da04bad7237be31fd,
title = "Chapter 15 Assay of Bacillus subtilis Ribonucleases In Vitro",
abstract = "Significant progress has been made recently regarding the identification of the ribonucleases involved in RNA maturation and degradation in Bacillus subtilis. More than half of these enzymes have no ortholog in Echerichia coli. To confirm that the in vivo effects of mutations in genes encoding RNases are direct, it is often necessary to purify the enzymes and assay their activity in vitro. Development of such assays is also necessary for detailed biochemical analysis of enzyme properties. In this chapter, we describe the purification and assay of 12 RNases of B. subtilis thought to be involved in stable RNA maturation or RNA degradation.",
author = "Ciar{\'a}n Condon and Olivier Pellegrini and Nathalie Mathy and Lionel B{\'e}nard and Yulia Redko and Oussenko, {Irina A.} and Gintaras Deikus and Bechhofer, {David H.}",
note = "Funding Information: We thank R. Hartmann, U. Mechold, and A. Gogos for providing details of the protocols for the isolation and assay of RNase P, Nano‐RNase, and EndoA, respectively. C. C. was supported by funds from the CNRS (UPR 9073), the Minist{\`e}re de l'Education Nationale, and the Agence Nationale de la Recherche (ANR). ",
year = "2008",
doi = "10.1016/S0076-6879(08)02215-5",
language = "English",
isbn = "9780123743770",
series = "Methods in Enzymology",
pages = "277--308",
editor = "Lynne Maquat and Cecilia Arraiano",
booktitle = "RNA Turnover in Prokaryotes, Archaea and Organelles",
}