Chapter 15 Assay of Bacillus subtilis Ribonucleases In Vitro

Ciarán Condon, Olivier Pellegrini, Nathalie Mathy, Lionel Bénard, Yulia Redko, Irina A. Oussenko, Gintaras Deikus, David H. Bechhofer

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

15 Scopus citations


Significant progress has been made recently regarding the identification of the ribonucleases involved in RNA maturation and degradation in Bacillus subtilis. More than half of these enzymes have no ortholog in Echerichia coli. To confirm that the in vivo effects of mutations in genes encoding RNases are direct, it is often necessary to purify the enzymes and assay their activity in vitro. Development of such assays is also necessary for detailed biochemical analysis of enzyme properties. In this chapter, we describe the purification and assay of 12 RNases of B. subtilis thought to be involved in stable RNA maturation or RNA degradation.

Original languageEnglish
Title of host publicationRNA Turnover in Prokaryotes, Archaea and Organelles
EditorsLynne Maquat, Cecilia Arraiano
Number of pages32
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879


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