Chapter 14 Analysis of mRNA Decay in Bacillus subtilis

David H. Bechhofer, Irina A. Oussenko, Gintaras Deikus, Shiyi Yao, Nathalie Mathy, Ciarán Condon

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

31 Scopus citations


Studies of mRNA turnover in B. subtilis are less well known than in E. coli. Here we provide researchers who have an interest in gram-positive RNA processing with several protocols for RNA isolation, for 5′- and 3′-mapping of mRNAs and mRNA decay fragments, and we also include a comprehensive listing of B. subtilis mutants that are deficient in ribonucleases thought to be involved in mRNA decay.

Original languageEnglish
Title of host publicationRNA Turnover in Prokaryotes, Archaea and Organelles
EditorsLynne Maquat, Cecilia Arraiano
Number of pages18
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879


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