TY - JOUR
T1 - Changes in the bacterial flora of the neoterminal ileum after ileocolonic resection for Crohn's disease
AU - Neut, Christel
AU - Bulois, Philippe
AU - Desreumaux, Pierre
AU - Membré, Jeanne Marie
AU - Lederman, Emmanuel
AU - Gambiez, Luc
AU - Cortot, Antoine
AU - Quandalle, Pierre
AU - Van Kruiningen, Herbert
AU - Colombel, Jean Frédéric
N1 - Funding Information:
Supported by INSERM (EPI 0114) and CH et U Lille (PHRC 1997).
PY - 2002
Y1 - 2002
N2 - OBJECTIVE: Bacterial agents have been implicated in the early recurrence of Crohn's disease after ileocolectomy. The aim of our study was to identify and quantify bacteria associated with the ileal mucosa in patients and controls and to correlate specific bacteria with recurrence. METHODS: The predominant bacterial microflora of the ileum were enumerated and identified, aerobically and anaerobically, in biopsies obtained at the time of surgery or by endoscopy from 61 patients with Crohn's disease and 10 ileocolectomy controls. The 61 specimens were comprised of 13 ileal biopsies taken from resection specimens, seven taken after ileostomy, and 41 taken after ileocolectomy. RESULTS: Ileocolectomy induced a significant increase in bacterial counts and variety in the neoterminal ileum in both patients and controls that was not observed in ileostomy biopsies. Comparison between patients and controls revealed greater numbers of Escherichia coli and enterococci in Crohn's disease and of bifidobacteria and ruminococci in controls. Early recurrence was associated with high counts of E. coli and bacteroides and the frequent isolation of fusobacteria. CONCLUSION: After ileocolectomy, colonization of the neoterminal ileum is increased. Our data suggest that increases in the populations of specific bacteria such as E. coli, enterococci, bacteroides, and fusobacteria may be important in postoperative recurrence of Crohn's disease.
AB - OBJECTIVE: Bacterial agents have been implicated in the early recurrence of Crohn's disease after ileocolectomy. The aim of our study was to identify and quantify bacteria associated with the ileal mucosa in patients and controls and to correlate specific bacteria with recurrence. METHODS: The predominant bacterial microflora of the ileum were enumerated and identified, aerobically and anaerobically, in biopsies obtained at the time of surgery or by endoscopy from 61 patients with Crohn's disease and 10 ileocolectomy controls. The 61 specimens were comprised of 13 ileal biopsies taken from resection specimens, seven taken after ileostomy, and 41 taken after ileocolectomy. RESULTS: Ileocolectomy induced a significant increase in bacterial counts and variety in the neoterminal ileum in both patients and controls that was not observed in ileostomy biopsies. Comparison between patients and controls revealed greater numbers of Escherichia coli and enterococci in Crohn's disease and of bifidobacteria and ruminococci in controls. Early recurrence was associated with high counts of E. coli and bacteroides and the frequent isolation of fusobacteria. CONCLUSION: After ileocolectomy, colonization of the neoterminal ileum is increased. Our data suggest that increases in the populations of specific bacteria such as E. coli, enterococci, bacteroides, and fusobacteria may be important in postoperative recurrence of Crohn's disease.
UR - http://www.scopus.com/inward/record.url?scp=0036117798&partnerID=8YFLogxK
U2 - 10.1016/S0002-9270(02)03969-2
DO - 10.1016/S0002-9270(02)03969-2
M3 - Article
C2 - 12003430
AN - SCOPUS:0036117798
SN - 0002-9270
VL - 97
SP - 939
EP - 946
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
IS - 4
ER -