Changes in Nup62 content affect contact-induced differentiation of cultured myoblasts

Patrick J. Bishop, Yayoi Kinoshita, N. Natalie Lopes, Avery S. Ward, D. Stave Kohtz

Research output: Contribution to journalArticlepeer-review

Abstract

Differentiation of cultured skeletal myoblasts is induced by extrinsic signals that include reduction in ambient mitogen concentration and increased cell density. Using an established murine myoblast cell line (C2C12), we have found that experimental reduction of the nucleoporin p62 (Nup62) content of myoblasts enhances differentiation in high-mitogen medium, while forced expression of Nup62 inhibits density-induced differentiation. In contrast, differentiation of myoblasts induced by low-mitogen medium was unaffected by ectopic Nup62 expression. Further analyses suggested that Nup62 content affects density-induced myoblast differentiation through a mechanism involving activation of p38 MAP kinase. Nuclear pore complex (NPC) composition, in particular changes in NUP62 content, may be altered during viral infection, differentiation, and in neoplastic growth. The results support a functional role for changes in Nup62 composition in NPCs and density-induced myogenic differentiation, and suggest a link between loss of Nup62 content and induction of an intracellular stress signaling pathways.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalDifferentiation
Volume114
DOIs
StatePublished - 1 Jul 2020

Keywords

  • Myoblast differentiation
  • Myogenesis
  • Nuclear pore complex
  • Nucleoporin p62 (Nup62)
  • p38 MAPK

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