Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: An in situ hybridization study

Toshiya Funabashi; Steven P. Kleopoulos; Philip J. Brooks; Fukuko Kimura; Donald W. Pfaff; Kazuyuki Shinohara; Charles V. Mobbs

doi:10.1016/S0168-0102(00)00150-4

Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: An in situ hybridization study

Toshiya Funabashi
, Steven P. Kleopoulos
, Philip J. Brooks
, Fukuko Kimura
, Donald W. Pfaff
, Kazuyuki Shinohara
, Charles V. Mobbs

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	85-92
Number of pages	8
Journal	Neuroscience Research
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/S0168-0102(00)00150-4
State	Published - 1 Sep 2000
Externally published	Yes

Keywords

Aging
Estrogen
Estrogen receptor α mRNA
In situ hybridization
Preoptic area
Progesterone receptor mRNA
Senescence
Ventromedial hypothalamus

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10.1016/S0168-0102(00)00150-4

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@article{fce72e83e5594110834b7c6b289d0bfc,

title = "Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: An in situ hybridization study",

abstract = "We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5\% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Aging, Estrogen, Estrogen receptor α mRNA, In situ hybridization, Preoptic area, Progesterone receptor mRNA, Senescence, Ventromedial hypothalamus",

author = "Toshiya Funabashi and Kleopoulos, \{Steven P.\} and Brooks, \{Philip J.\} and Fukuko Kimura and Pfaff, \{Donald W.\} and Kazuyuki Shinohara and Mobbs, \{Charles V.\}",

note = "Funding Information: We are very grateful to Dr Junzo Kato, Yamanashi Medical School (Yamanashi, Japan), for providing rat PR cDNA. The present study was supported by the American Federation for Aging Research and the Glenn Foundation, of which C.V.M. was a fellow, and a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science, Sports and Culture to T.F.",

year = "2000",

month = sep,

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doi = "10.1016/S0168-0102(00)00150-4",

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Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: An in situ hybridization study. / Funabashi, Toshiya; Kleopoulos, Steven P.; Brooks, Philip J. et al.
In: Neuroscience Research, Vol. 38, No. 1, 01.09.2000, p. 85-92.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging

T2 - An in situ hybridization study

AU - Funabashi, Toshiya

AU - Kleopoulos, Steven P.

AU - Brooks, Philip J.

AU - Kimura, Fukuko

AU - Pfaff, Donald W.

AU - Shinohara, Kazuyuki

AU - Mobbs, Charles V.

N1 - Funding Information: We are very grateful to Dr Junzo Kato, Yamanashi Medical School (Yamanashi, Japan), for providing rat PR cDNA. The present study was supported by the American Federation for Aging Research and the Glenn Foundation, of which C.V.M. was a fellow, and a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science, Sports and Culture to T.F.

PY - 2000/9/1

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N2 - We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.

AB - We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.

KW - Aging

KW - Estrogen

KW - Estrogen receptor α mRNA

KW - In situ hybridization

KW - Preoptic area

KW - Progesterone receptor mRNA

KW - Senescence

KW - Ventromedial hypothalamus

UR - https://www.scopus.com/pages/publications/0034281988

U2 - 10.1016/S0168-0102(00)00150-4

DO - 10.1016/S0168-0102(00)00150-4

M3 - Article

C2 - 10997581

AN - SCOPUS:0034281988

SN - 0168-0102

VL - 38

SP - 85

EP - 92

JO - Neuroscience Research

JF - Neuroscience Research

IS - 1

ER -

Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: An in situ hybridization study

Abstract

Keywords

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