TY - JOUR
T1 - Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging
T2 - An in situ hybridization study
AU - Funabashi, Toshiya
AU - Kleopoulos, Steven P.
AU - Brooks, Philip J.
AU - Kimura, Fukuko
AU - Pfaff, Donald W.
AU - Shinohara, Kazuyuki
AU - Mobbs, Charles V.
N1 - Funding Information:
We are very grateful to Dr Junzo Kato, Yamanashi Medical School (Yamanashi, Japan), for providing rat PR cDNA. The present study was supported by the American Federation for Aging Research and the Glenn Foundation, of which C.V.M. was a fellow, and a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science, Sports and Culture to T.F.
PY - 2000/9/1
Y1 - 2000/9/1
N2 - We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.
AB - We examined two molecular responses to estrogen, reduction in estrogen receptor α (ERα) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17β-estradiol, or empty implants, and killed 4 days after implantation. By means of in situ hybridization, we found that, in young rats, estrogen reduced ERα mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ERα mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ERα mRNA in the VMH and ARC was significantly impaired with age, but not in the POA. (C) 2000 Elsevier Science Ireland Ltd.
KW - Aging
KW - Estrogen
KW - Estrogen receptor α mRNA
KW - In situ hybridization
KW - Preoptic area
KW - Progesterone receptor mRNA
KW - Senescence
KW - Ventromedial hypothalamus
UR - http://www.scopus.com/inward/record.url?scp=0034281988&partnerID=8YFLogxK
U2 - 10.1016/S0168-0102(00)00150-4
DO - 10.1016/S0168-0102(00)00150-4
M3 - Article
C2 - 10997581
AN - SCOPUS:0034281988
SN - 0168-0102
VL - 38
SP - 85
EP - 92
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -