Chain initiation by reovirus RNA transcriptase in vitro

Daniel H. Levin, George Acs, Samuel C. Silvebstein

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

THE presence of reovirus RNA transcriptase within the virion has facilitated studies on the mechanism of mRNA synthesis in vitro1,2. Subviral particles prepared from extracts of reovirus infected L-929 cells have provided stable and highly active RNA transcriptase preparations3. The particulate enzyme efficiently synthesizes in vitro all ten species of the reovirus-specific single-stranded mRNAs that correspond to the ten segments of the double-stranded RNA genome3,5. The same transcription mechanism seems to apply in vivo and in vitro as suggested by (a) the correlation in size and distribution of the messenger species3-8 and (b) the complementarity of the corresponding mRNAs to the same template strand (unpublished data of D. Kyner).

Original languageEnglish
Pages (from-to)603-604
Number of pages2
JournalNature
Volume227
Issue number5258
DOIs
StatePublished - 1970
Externally publishedYes

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