Ceramide as an activator lipid of cathepsin D

Michael Heinrich; Marc Wickel; Supandi Winoto-Morbach; Wulf Schneider-Brachert; Thomas Weber; Josef Brunner; Paul Saftig; Christoph Peters; Martin Krönke; Stefan Schütze

Ceramide as an activator lipid of cathepsin D

Michael Heinrich, Marc Wickel, Supandi Winoto-Morbach, Wulf Schneider-Brachert, Thomas Weber, Josef Brunner, Paul Saftig, Christoph Peters, Martin Krönke, Stefan Schütze

Research output: Contribution to journal › Article › peer-review

103 Scopus citations

Abstract

We have identified the aspartic protease cathepsin D as a novel intracellular target protein for the lipid second messenger ceramide. Ceramide specifically binds to and induces CTSD proteolytic activity. A-SMase deficient cells derived from Niemann-Pick patients show decreased CTSD activity that was reconstituted by transfection with A-SMase cDNA. Ceramide accumulation in cells derived from A-ceramidase defective Farber patients correlates with enhanced CTSD activity. These findings suggest that A-SMase-derived ceramide targets endolysosomal CTSD.

Original language	English
Pages (from-to)	305-315
Number of pages	11
Journal	Advances in Experimental Medicine and Biology
Volume	477
State	Published - 2000
Externally published	Yes

Keywords

Acid Sphingomyelinase
Cathepsin D
Ceramide
TID-Ceramide

Cite this

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title = "Ceramide as an activator lipid of cathepsin D",

abstract = "We have identified the aspartic protease cathepsin D as a novel intracellular target protein for the lipid second messenger ceramide. Ceramide specifically binds to and induces CTSD proteolytic activity. A-SMase deficient cells derived from Niemann-Pick patients show decreased CTSD activity that was reconstituted by transfection with A-SMase cDNA. Ceramide accumulation in cells derived from A-ceramidase defective Farber patients correlates with enhanced CTSD activity. These findings suggest that A-SMase-derived ceramide targets endolysosomal CTSD.",

keywords = "Acid Sphingomyelinase, Cathepsin D, Ceramide, TID-Ceramide",

author = "Michael Heinrich and Marc Wickel and Supandi Winoto-Morbach and Wulf Schneider-Brachert and Thomas Weber and Josef Brunner and Paul Saftig and Christoph Peters and Martin Kr{\"o}nke and Stefan Sch{\"u}tze",

year = "2000",

language = "English",

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pages = "305--315",

journal = "Advances in Experimental Medicine and Biology",

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TY - JOUR

T1 - Ceramide as an activator lipid of cathepsin D

AU - Heinrich, Michael

AU - Wickel, Marc

AU - Winoto-Morbach, Supandi

AU - Schneider-Brachert, Wulf

AU - Weber, Thomas

AU - Brunner, Josef

AU - Saftig, Paul

AU - Peters, Christoph

AU - Krönke, Martin

AU - Schütze, Stefan

PY - 2000

Y1 - 2000

N2 - We have identified the aspartic protease cathepsin D as a novel intracellular target protein for the lipid second messenger ceramide. Ceramide specifically binds to and induces CTSD proteolytic activity. A-SMase deficient cells derived from Niemann-Pick patients show decreased CTSD activity that was reconstituted by transfection with A-SMase cDNA. Ceramide accumulation in cells derived from A-ceramidase defective Farber patients correlates with enhanced CTSD activity. These findings suggest that A-SMase-derived ceramide targets endolysosomal CTSD.

AB - We have identified the aspartic protease cathepsin D as a novel intracellular target protein for the lipid second messenger ceramide. Ceramide specifically binds to and induces CTSD proteolytic activity. A-SMase deficient cells derived from Niemann-Pick patients show decreased CTSD activity that was reconstituted by transfection with A-SMase cDNA. Ceramide accumulation in cells derived from A-ceramidase defective Farber patients correlates with enhanced CTSD activity. These findings suggest that A-SMase-derived ceramide targets endolysosomal CTSD.

KW - Acid Sphingomyelinase

KW - Cathepsin D

KW - Ceramide

KW - TID-Ceramide

UR - http://www.scopus.com/inward/record.url?scp=0034470251&partnerID=8YFLogxK

M3 - Article

C2 - 10849758

AN - SCOPUS:0034470251

SN - 0065-2598

VL - 477

SP - 305

EP - 315

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Ceramide as an activator lipid of cathepsin D

Abstract

Keywords

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