Centromere sequences localize to the nuclear halo of human spermatozoa

Y. Yaron, J. A. Kramer, K. Gyi, S. A.D. Ebrahim, M. I. Evans, M. P. Johnson, S. A. Krawetz

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations

Abstract

Chromatin is organized into a series of discrete nuclear matrix-associated and non-nuclear matrix-associated domains. The non-matrix-associated domains consist of loops of DNA that are attached to the proteinaceous nuclear matrix by matrix-associated regions (MARs). Although this organization is well characterized in somatic cells, comparatively little is known of this mode of organizing the genome in the human sperm nucleus. To define this relationship, the interaction of human sperm chromatin with the nuclear matrix was assessed by fluorescence in situ hybridization using specific alpha satellite probes directed to the centromeric regions of chromosomes 13 plus 21 and 18. Hybridization of the centromeric sequences was visualized as segmented, bungled structures that extended from the nuclear core into the halo.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalInternational Journal of Andrology
Volume21
Issue number1
DOIs
StatePublished - 1998

Keywords

  • Fluorescence in situ hybridization
  • Human spermatozoa
  • Matrix-associated regions
  • Nuclear halos
  • Nuclear matrix

Fingerprint

Dive into the research topics of 'Centromere sequences localize to the nuclear halo of human spermatozoa'. Together they form a unique fingerprint.

Cite this