TY - JOUR
T1 - Cellular resolution anatomical and molecular atlases for prenatal human brains
AU - Ding, Song Lin
AU - Royall, Joshua J.
AU - Lesnar, Phil
AU - Facer, Benjamin A.C.
AU - Smith, Kimberly A.
AU - Wei, Yina
AU - Brouner, Kristina
AU - Dalley, Rachel A.
AU - Dee, Nick
AU - Dolbeare, Tim A.
AU - Ebbert, Amanda
AU - Glass, Ian A.
AU - Keller, Nika H.
AU - Lee, Felix
AU - Lemon, Tracy A.
AU - Nyhus, Julie
AU - Pendergraft, Julie
AU - Reid, Robert
AU - Sarreal, Melaine
AU - Shapovalova, Nadiya V.
AU - Szafer, Aaron
AU - Phillips, John W.
AU - Sunkin, Susan M.
AU - Hohmann, John G.
AU - Jones, Allan R.
AU - Hawrylycz, Michael J.
AU - Hof, Patrick R.
AU - Ng, Lydia
AU - Bernard, Amy
AU - Lein, Ed S.
N1 - Publisher Copyright:
© 2021 Wiley Periodicals LLC
PY - 2022/1
Y1 - 2022/1
N2 - Increasing interest in studies of prenatal human brain development, particularly using new single-cell genomics and anatomical technologies to create cell atlases, creates a strong need for accurate and detailed anatomical reference atlases. In this study, we present two cellular-resolution digital anatomical atlases for prenatal human brain at postconceptional weeks (PCW) 15 and 21. Both atlases were annotated on sequential Nissl-stained sections covering brain-wide structures on the basis of combined analysis of cytoarchitecture, acetylcholinesterase staining, and an extensive marker gene expression dataset. This high information content dataset allowed reliable and accurate demarcation of developing cortical and subcortical structures and their subdivisions. Furthermore, using the anatomical atlases as a guide, spatial expression of 37 and 5 genes from the brains, respectively, at PCW 15 and 21 was annotated, illustrating reliable marker genes for many developing brain structures. Finally, the present study uncovered several novel developmental features, such as the lack of an outer subventricular zone in the hippocampal formation and entorhinal cortex, and the apparent extension of both cortical (excitatory) and subcortical (inhibitory) progenitors into the prenatal olfactory bulb. These comprehensive atlases provide useful tools for visualization, segmentation, targeting, imaging, and interpretation of brain structures of prenatal human brain, and for guiding and interpreting the next generation of cell census and connectome studies.
AB - Increasing interest in studies of prenatal human brain development, particularly using new single-cell genomics and anatomical technologies to create cell atlases, creates a strong need for accurate and detailed anatomical reference atlases. In this study, we present two cellular-resolution digital anatomical atlases for prenatal human brain at postconceptional weeks (PCW) 15 and 21. Both atlases were annotated on sequential Nissl-stained sections covering brain-wide structures on the basis of combined analysis of cytoarchitecture, acetylcholinesterase staining, and an extensive marker gene expression dataset. This high information content dataset allowed reliable and accurate demarcation of developing cortical and subcortical structures and their subdivisions. Furthermore, using the anatomical atlases as a guide, spatial expression of 37 and 5 genes from the brains, respectively, at PCW 15 and 21 was annotated, illustrating reliable marker genes for many developing brain structures. Finally, the present study uncovered several novel developmental features, such as the lack of an outer subventricular zone in the hippocampal formation and entorhinal cortex, and the apparent extension of both cortical (excitatory) and subcortical (inhibitory) progenitors into the prenatal olfactory bulb. These comprehensive atlases provide useful tools for visualization, segmentation, targeting, imaging, and interpretation of brain structures of prenatal human brain, and for guiding and interpreting the next generation of cell census and connectome studies.
UR - http://www.scopus.com/inward/record.url?scp=85119297333&partnerID=8YFLogxK
U2 - 10.1002/cne.25243
DO - 10.1002/cne.25243
M3 - Article
C2 - 34525221
AN - SCOPUS:85119297333
SN - 0021-9967
VL - 530
SP - 6
EP - 503
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 1
ER -