Cellular origin of the transforming gene of Moloney murine sarcoma virus

E. Canaani, S. R. Tronick, K. C. Robbins, P. R. Andersen, C. Y. Dunn, S. A. Aaronson

Research output: Contribution to journalArticlepeer-review

Abstract

The replication-defective nature of mammalian sarcoma viruses has made genetic analysis of these viruses very difficult. Thus, it has not been possible to identify the region of the viral genome necessary for malignant transformation. Recently, the application of restriction endonuclease technology has led to the generation of a physical map of M-MuSV. In this paper, we have taken advantage of this biochemical approach to identify the portion of the viral genome that can be deleted either in a naturally occurring mutant or in a restriction enzyme cleavage product without affecting the transforming ability of the viral genome. By this strategy, it has been possible to localize the transformation-inducing information of M-MuSV. We have also applied recombinant DNA techniques to the analysis of this viral genome. It has been possible to clone the M-MuSV genome and to study the arrangement of its cell-derived transforming sequences within normal mouse cell DNA.

Original languageEnglish
Pages (from-to)727-734
Number of pages8
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume44
Issue number2
StatePublished - 1979

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