@article{999bf7ff071c40048fb848b317f6249a,
title = "Cellular detection of multiple antigens at single cell resolution using antibodies generated from the same species",
abstract = "Biology at a cellular level comes with a great amount of heterogeneity. It is now evident that even clonally propagated cells in an in vitro population do not express the same set of cellular epitopes. The vascular endothelial as well as blood cells show a very high degree of heterogeneity in expression of specific proteins. Although several methods exist for identification of genome or transcriptome from a single cell, there is still limited advancement in detection of multiple cellular antigens in a single cell. This has been mainly due to the limited availability of different antibodies. Single-cell detection methods involving the use of multiple monoclonal antibodies generated in the same species would therefore provide with an important tool for cellular detection of antigens. Here, we describe a method to assess multiple proteins in a cell using different antibodies generated in the same species.",
keywords = "Antibody, Antigen, Di-thiothreitol (DTT), Fluorophores, Stripping buffer",
author = "Ranjan, {Amaresh Kumar} and Joglekar, {Mugdha V.} and Atre, {Ashwini N.} and Milind Patole and Bhonde, {Ramesh R.} and Hardikar, {Anandwardhan A.}",
note = "Funding Information: The authors thank Dr. GC Mishra, Director, NCCS and Dr. Milind Patole, NCCS for support. AAH acknowledges the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program obtained at the O'Brien Institute. AKR acknowledges the support of senior research fellowship from the Council of Scientific and Industrial Research, Government of India . This work was supported by funding from the National Center for Cell Science, Department of Biotechnology, Government of India ( BT/PR7975/MED/14/1211/2006 ) to AAH. MVJ is presently supported by the Juvenile Diabetes Research Foundation postdoctoral award (JDRF 3-2011-352 ). AAH is supported by Australian Future Fellowship ( FT110100254 ) through the Australian Research Council.",
year = "2012",
month = may,
day = "31",
doi = "10.1016/j.jim.2012.02.018",
language = "English",
volume = "379",
pages = "42--47",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier B.V.",
number = "1-2",
}