Cell wall sorting of lipoproteins in Staphylococcus aureus

William Wiley Navarre, Simon Daefler, Olaf Schneewind

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Many surface proteins are thought to be anchored to the cell wall of gram- positive organisms via their C termini, while the N-terminal domains of these molecules are displayed on the bacterial surface. Cell wall anchoring of surface proteins in Staphylococcus aureus requires both an N-terminal leader peptide and a C-terminal cell wall sorting signal. By fusing the cell wall sorting signal of protein A to the C terminus of staphylococcal β-lactamase, we demonstrate here that lipoproteins can also be anchored to the cell wall of S. aureus. The topology of cell wall-anchored β-lactamase is reminiscent of that described for Braun's murein lipoprotein in that the N terminus of the polypeptide chain is membrane anchored whereas the C-terminal end is tethered to the bacterial cell wall.

Original languageEnglish
Pages (from-to)441-446
Number of pages6
JournalJournal of Bacteriology
Issue number2
StatePublished - Jan 1996
Externally publishedYes


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