Abstract
In this chapter, we describe the ultrastructure of Chlamydomonas reinhardtii, with some examples from Chlamydomonas eugametos and Chlamydomonas monoica. Our ultrastructural tour survey images obtained by classic thin-sectioning of fixed material and by two techniques that visualize frozen nonfixed cells: quick-freeze deep-etch electron microscopy and cryo-electron tomography. Particular attention is given to the structure of the nucleus, endoplasmic reticulum, Golgi, contractile vacuoles, acidocalcisomes, microbodies, cytoplasmic vacuoles, and mitochondria.
| Original language | English |
|---|---|
| Title of host publication | The Chlamydomonas Sourcebook |
| Subtitle of host publication | Volume 1: Introduction to Chlamydomonas and Its Laboratory Use |
| Publisher | Elsevier |
| Pages | 17-40 |
| Number of pages | 24 |
| Volume | 1 |
| ISBN (Electronic) | 9780128224571 |
| ISBN (Print) | 9780128224588 |
| DOIs | |
| State | Published - 1 Jan 2023 |
| Externally published | Yes |
Keywords
- Chlamydomonas
- acidocalcisome
- autophagy
- contractile vacuole
- cryo-ET
- microsomes
- mitochondria
- nuclear pore
- quick-freeze deep-etch EM
- vacuole