Cell membrane potential: a signal to control intracellular pH and transepithelial hydrogen ion secretion in frog kidney

W. Wang, P. Dietl, S. Silbernagl, H. Oberleithner

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The dependence of intracellular pH (pHi) and transepithelial H+ secretion on the cell membrane potential (Vm) was tested applying pH-sensitive and conventional microelectrodes in giant cells fused from single epithelial cells of the diluting segment and in intact tubules of the frog kidney. An increase of extracellular K+ concentration from 3 to 15 mmol/l decreased Vm from -49±4 to -29±1 mV while pHi increased from 7.44±0.04 to 7.61±0.06. Addition of 1 mmol/l Ba2+ depolarized Vm from -45±3 to -32±2 mV, paralleled by an increase of pHi from 7.46±0.04 to 7.58±0.03. Application of 0.05 mmol/l furosemide hyperpolarized Vm from -48±3 to -53±3 mV and decreased pHi from 7.47±0.05 to 7.42±0.05. In the intact diluting segment of the isolated-perfused frog kidney an increase of peritubular K+ concentration from 3 to 15 mmol/l increased the luminal pH from 7.23±0.08 to 7.41±0.08. Addition of Ba2+ to the peritubular perfusate also increased luminal pH from 7.35±0.07 to 7.46±0.07. Addition of furosemide decreased luminal pH from 7.32±0.03 to 7.24±0.05. We conclude: cell depolarization reduces the driving force for the rheogenic HCO3- exit step across the basolateral cell membrane. HCO3- accumulates in the cytoplasm and pHi increases. An alkaline pHi inactivates the luminal Na+/H+ exchanger. This diminishes transepithelial H+ secretion. Cell hyperpolarization leads to the opposite phenomenon. Thus, pHi serves as signal transducer between cell voltage and Na+/H+ exchange.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume409
Issue number3
DOIs
StatePublished - Jul 1987
Externally publishedYes

Keywords

  • Cell fusion
  • Cell membrane potential
  • Diluting segment
  • Frog kidney
  • Intracellular pH

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