Cell-free reconstitution of β -amyloid production and trafficking

Dongming Cai, William J. Netzer, Feng Li, Huaxi Xu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The production of β -amyloid is one aspect of the metabolism of β APP. To achieve an effective understanding of this process, its must be considered in terms of both the enzymatic activities required for the proteolytic processing of β APP and the trafficking of β APP and its metabolites within the secretory pathway. Whole cells are often insufficient to elucidate the details of this process. We have therefore developed a cell-free reconstitution system for β -amyloid and β APP production and trafficking. This experimental system has allowed us to study the effects of adding or removing salient cellular factors and to focus on β APP processing as it occurs in various subcellular compartments.

Original languageEnglish
Title of host publicationAmyloid Precursor Protein
Subtitle of host publicationA Practical Approach
PublisherCRC Press
Pages69-81
Number of pages13
ISBN (Electronic)9780203492185
ISBN (Print)9780849322457
StatePublished - 1 Jan 2004
Externally publishedYes

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