Abstract
The production of β -amyloid is one aspect of the metabolism of β APP. To achieve an effective understanding of this process, its must be considered in terms of both the enzymatic activities required for the proteolytic processing of β APP and the trafficking of β APP and its metabolites within the secretory pathway. Whole cells are often insufficient to elucidate the details of this process. We have therefore developed a cell-free reconstitution system for β -amyloid and β APP production and trafficking. This experimental system has allowed us to study the effects of adding or removing salient cellular factors and to focus on β APP processing as it occurs in various subcellular compartments.
Original language | English |
---|---|
Title of host publication | Amyloid Precursor Protein |
Subtitle of host publication | A Practical Approach |
Publisher | CRC Press |
Pages | 69-81 |
Number of pages | 13 |
ISBN (Electronic) | 9780203492185 |
ISBN (Print) | 9780849322457 |
State | Published - 1 Jan 2004 |
Externally published | Yes |