Cell-free human amniotic fluid as culture medium for mouse and human embryos

A. D. Dorfmann, S. D. Bender, P. Robinson, E. F. Fugger, M. Bustillo, G. Reed, J. D. Schulman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Differences between murine and human preimplantation embryonic growth in cell-free human amniotic fluid and medium F-10 supplemented with human fetal cord serum were assessed. Two-cell mouse embryos cultured in human amniotic fluid developed more consistently to the expanded blastocyst stage than those cultured in F-10. No significant difference in human preimplantation embryo cleavage was observed between the two culture conditions. However, amniotic fluid was significantly worse than F-10 + human serum in its ability to maintain embryos that could establish pregnancy after transfer.

Original languageEnglish
Pages (from-to)671-674
Number of pages4
JournalFertility and Sterility
Issue number4
StatePublished - 1989
Externally publishedYes


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