Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ

Andrea D. Branch, Hugh D. Robertson, Christopher Greer, Peter Gegenheimer, Craig Peebles, John Abelson

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ. Linear viroid molecules are efficiently converted to circular molecules (circles) which are indistinguishable by electrophoretic mobility and two-dimensional oligonucleotide pattern from viroid circles extracted from infected plants. In light of recent evidence for multimeric viroid replication intermediates, cleavage followed by RNA ligation by a cellular enzyme may (i) be a normal step in the viroid life cycle and (ii) may also reflect cellular events.

Original languageEnglish
Pages (from-to)1147-1149
Number of pages3
JournalScience
Volume217
Issue number4565
DOIs
StatePublished - 1982
Externally publishedYes

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