Cell finder speeds impalements with microelectrodes

William S. Marshall; Stephen D. Klyce

doi:10.1007/BF00596181

Cell finder speeds impalements with microelectrodes

William S. Marshall, Stephen D. Klyce

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple circuit is described, which, in conjunction with an electrometer and a digitally-controlled hydraulic microdrive, automatically advances a microelectrode through the bathing solution and halts the microelectrode when a cell is impaled. The distance between initial contact with the cell membrane and cell penetration (1-5 μm for rabbit corneal epithelium) is dependent upon the physical characteristics of the cell and microelectrode, and can be optimized by empirical adjustment.

Original language	English
Pages (from-to)	258-259
Number of pages	2
Journal	Pflugers Archiv European Journal of Physiology
Volume	391
Issue number	3
DOIs	https://doi.org/10.1007/BF00596181
State	Published - Sep 1981
Externally published	Yes

Keywords

Corneal epithelium
Hydraulic microdrive
Intracellular recording
Microelectrode

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10.1007/BF00596181

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abstract = "A simple circuit is described, which, in conjunction with an electrometer and a digitally-controlled hydraulic microdrive, automatically advances a microelectrode through the bathing solution and halts the microelectrode when a cell is impaled. The distance between initial contact with the cell membrane and cell penetration (1-5 μm for rabbit corneal epithelium) is dependent upon the physical characteristics of the cell and microelectrode, and can be optimized by empirical adjustment.",

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AU - Klyce, Stephen D.

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AB - A simple circuit is described, which, in conjunction with an electrometer and a digitally-controlled hydraulic microdrive, automatically advances a microelectrode through the bathing solution and halts the microelectrode when a cell is impaled. The distance between initial contact with the cell membrane and cell penetration (1-5 μm for rabbit corneal epithelium) is dependent upon the physical characteristics of the cell and microelectrode, and can be optimized by empirical adjustment.

KW - Corneal epithelium

KW - Hydraulic microdrive

KW - Intracellular recording

KW - Microelectrode

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JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

IS - 3

ER -

Cell finder speeds impalements with microelectrodes

Abstract

Keywords

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